BUBR1 Antibody (YA2757)(PBS only)

Cat. No.: HY-P83012A: Data Sheet User Guide for Antibodies
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BUBR1 Antibody (YA2757) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BUBR1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

BUBR1 Antibody (YA2757) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BUBR1.

Host

Rabbit

Molecular Weight

Predicted band size: 120 kDa;

Observed band size: 120 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O60566

Gene ID

701 [NCBI]

Immunogen

A synthetic peptide of human BubR1 aa48-237.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:500
IP IP: Immunoprecipitation	1:20-1:50

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression

Subcellular Localization：Cytoplasm; Nucleus; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Expression：
Tissue_specificity:Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index

Induction:Induced during mitosis

Isoforms & Post-Translational Modification：O60566 has 3 isomers: O60566-1: 119545 Da (predicted); O60566-2: 105890 Da (predicted); O60566-3: 121386 Da (predicted).
Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610;Acetylation at Lys-250 regulates its degradation and timing in anaphase entry;Ubiquitinated. Degraded by the proteasome (PubMed:19407811). Ubiquitinated by UBR5, promoting disassembly of the mitotic checkpoint complex from the APC/C complex (PubMed:35217622);Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore;Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase

Subunit：Interacts with CENPE (PubMed:12925705, PubMed:19625775). Interacts with PLK1 (PubMed:16760428, PubMed:17376779, PubMed:17785528, PubMed:19503101). Part of a complex containing BUB3, CDC20 and BUB1B (PubMed:11702782). Interacts with anaphase-promoting complex/cyclosome (APC/C) (PubMed:10477750). Interacts with KNL1 (PubMed:17981135). Interacts with KAT2B (PubMed:19407811). Interacts with RIPK3 (PubMed:29883609). Interacts with the closed conformation form of MAD2L1 (PubMed:29162720)

Research Field

Cell Biology

Synonyms

BUB1B; BUBR1; MAD3L; SSK1; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1

Documentation

User Guide for Antibodies (1077 KB)