HN1 Antibody (YA6595)

Cat. No.: HY-P86902: Data Sheet COA
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User Guide for Antibodies
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HN1 Antibody (YA6595) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HN1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

HN1 Antibody (YA6595) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HN1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 16 kDa;

Observed band size: 20 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

Q9UK76

Gene ID

51155 [NCBI]

Immunogen

Synthetic peptide within human HN1 aa 105-154/154.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Modulates negatively AKT-mediated GSK3B signaling (PubMed:21323578, PubMed:22155408). Induces CTNNB1 'Ser-33' phosphorylation and degradation through the suppression of the inhibitory 'Ser-9' phosphorylation of GSK3B, which represses the function of the APC:CTNNB1:GSK3B complex and the interaction with CDH1/E-cadherin in adherent junctions (PubMed:25169422). Plays a role in the regulation of cell cycle and cell adhesion (PubMed:25169422, PubMed:25450365). Has an inhibitory role on AR-signaling pathway through the induction of receptor proteasomal degradation (PubMed:22155408)

Subcellular Localization：Nucleus,Cytoplasm

Expression：
Tissue_Specificity: Expressed in testis, skeletal muscle, thymus, prostate, colon, peripheral blood cells, brain and placenta
Induction: Induced by EGF growth factor at mRNA and protein levels (PubMed:21323578). Induced by androgens (PubMed:22155408). Negatively regulated by the microRNA miR-132 (PubMed:25450365)

Isoforms & Post-Translational Modification：Q9UK76 has three isomers: Q9UK76-1: 16015 Da (predicted); Q9UK76-2: 19916 Da (predicted); Q9UK76-3: 11023 Da (predicted).

Subunit：Interacts with the complex composed, at least, of APC, CTNNB1 and GSK3B; the interaction takes place with the inactive form of GSK3B (phosphorylated at 'Ser-9')

Synonyms

Androgen regulated protein 2 antibody; Androgen-regulated protein 2 antibody; ARM2 antibody; Hematological and neurological expressed 1 antibody; Hematological and neurological expressed 1 protein antibody; HN1 antibody; HN1_HUMAN antibody; HN1A antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)