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EWSR1 Antibody (YA2270)

(Synonyms: EWSR1; EWS; RNA-binding protein EWS; EWS oncogene; Ewing sarcoma breakpoint region 1 protein)
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Based on 1 Customer Validation

EWSR1 Antibody (YA2270) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EWSR1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • EWSR1 Antibody (YA2270) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

EWSR1 Antibody (YA2270) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EWSR1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 85 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human EWSR1 aa100-162/656.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104631

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EWSR1 Antibody (YA2270)
    Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using EWSR1 (HY-P82525) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
    Immunocytochemistry analysis of A431 cells labeling EWSR1 Antibody (HY-P82525) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with EWSR1 Antibody (HY-P82525) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
    Immunocytochemistry analysis of SK-BR-3 cells labeling EWSR1 Antibody (HY-P82525) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with EWSR1 Antibody (HY-P82525) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
    Immunohistochemical analysis of paraffin-embedded Rat testis tissue using EWSR1 Antibody (YA2270). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82525, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
    Immunohistochemical analysis of paraffin-embedded Rat testis tissue using EWSR1 Antibody (YA2270). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82525, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    EWSR1 binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes[1][2].

  • Subcellular Localization

    Nucleus; Cytoplasm; Cell membrane

  • Expression


    Tissue_specificity:Ubiquitous

  • Isoforms & Post-Translational Modification

    Q01844 has 6 isomers: Q01844-1: 68478 Da (predicted); Q01844-2: 61217 Da (predicted); Q01844-3: 68391 Da (predicted); Q01844-4: 37620 Da (predicted); Q01844-5: 68966 Da (predicted); Q01844-6: 62508 Da (predicted).
    Phosphorylated; calmodulin-binding inhibits phosphorylation of Ser-266;Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8

  • Subunit

    Binds POLR2C, SF1, calmodulin and RNA. Interacts with PTK2B/FAK2 and TDRD3. Binds calmodulin in the presence, but not in the absence, of calcium ion. Forms a complex with REC8, PRDM9, SYCP3 and SYCP1; complex formation is dependent of phosphorylated form of REC8 and requires PRDM9 bound to hotspot DNA; EWSR1 joins PRDM9 with the chromosomal axis through REC8 (By similarity)

  • SwissProt ID

    Q01844

  • Gene ID
    2130 [NCBI]
  • Synonyms

    EWSR1; EWS; RNA-binding protein EWS; EWS oncogene; Ewing sarcoma breakpoint region 1 protein

  • Research Field

    Tags & Cell Markers

EWSR1 Antibody (YA2270) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using EWSR1 (HY-P82525) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling EWSR1 Antibody (HY-P82525) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with EWSR1 Antibody (HY-P82525) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of SK-BR-3 cells labeling EWSR1 Antibody (HY-P82525) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with EWSR1 Antibody (HY-P82525) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat testis tissue using EWSR1 Antibody (YA2270). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82525, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat testis tissue using EWSR1 Antibody (YA2270). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82525, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EWSR1 Antibody (YA2270) Product Image
    1/16
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • EWSR1 Antibody (YA2270) Product Image
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using EWSR1 (HY-P82525) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling EWSR1 Antibody (HY-P82525) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with EWSR1 Antibody (HY-P82525) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of SK-BR-3 cells labeling EWSR1 Antibody (HY-P82525) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with EWSR1 Antibody (HY-P82525) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat testis tissue using EWSR1 Antibody (YA2270). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82525, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat testis tissue using EWSR1 Antibody (YA2270). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82525, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • Experimental Validation Results for EWSR1 Antibody (YA2270)
  • EWSR1 Antibody (YA2270) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using EWSR1 (HY-P82525) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • EWSR1 Antibody (YA2270) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling EWSR1 Antibody (HY-P82525) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with EWSR1 Antibody (HY-P82525) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • EWSR1 Antibody (YA2270) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of SK-BR-3 cells labeling EWSR1 Antibody (HY-P82525) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with EWSR1 Antibody (HY-P82525) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • EWSR1 Antibody (YA2270) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue using EWSR1 Antibody (YA2270). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82525, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EWSR1 Antibody (YA2270) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue using EWSR1 Antibody (YA2270). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82525, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EWSR1 Antibody (YA2270) Product Image WB
  • EWSR1 Antibody (YA2270) Product Image ICC
  • EWSR1 Antibody (YA2270) Product Image ICC
  • EWSR1 Antibody (YA2270) Product Image IHC-P
  • EWSR1 Antibody (YA2270) Product Image IHC-P