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CD68 Antibody (YA798)

(Synonyms: CD 68 antibody; CD68 antibody; CD68 antigen antibody; CD68 molecule antibody; CD68_HUMAN antibody; DKFZp686M18236 antibody; gp11 antibody; Gp110 antibody; LAMP4 antibody; Macrophage antigen CD68 (microsialin) antibody; MACROPHAGE ANTIGEN CD68 antibody; Macrosialin antibody; SCARD1 antibody; Scavenger receptor class D member 1 antibody)
Customer Review

Based on 1 Customer Validation

CD68 Antibody (YA798) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD68.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • CD68 Antibody (YA798) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:50-1:200 1:50-1:100

Product Details

Description

CD68 Antibody (YA798) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD68.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 75-130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 37 kDa；
Species Reactivity Database

Entrez Gene: 968 Human

SwissProt: P34810 Human

Immunogen

Synthetic peptide corresponding to Human CD68.AA range:320-354.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102065

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD68 Antibody (YA798)
    Western blot analysis of extracts from THP-1 (lane 1) and HepG2 (lane 2) and RAW264.7 (lane 3) using CD68 Antibody (YA798) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for CD68 Antibody (YA798)
    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD68 Antibody (YA798)
    Immunohistochemical analysis of paraffin-embedded human non-hodgkin lymphoma using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD68 Antibody (YA798)
    Immunohistochemical analysis of paraffin-embedded human head and neck cancer using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD68 Antibody (YA798)
    Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD68 Antibody (YA798)
    Flow cytometric analysis of 1X106 THP-1 cells labeling CD68 Antibody (HY-P80066, red). Cells were stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD68 Antibody (YA798)
    Flow cytometric analysis of 1X106 THP-1 cells labeling CD68 Antibody (HY-P80066, red). Cells were stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CD68: This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Endosome membrane; Single-pass type I membrane protein; Lysosome membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is highly expressed in blood monocytes and tissue macrophages. It is also expressed in lymphocytes, fibroblasts, and endothelial cells. It is expressed in many tumor cell lines, which may enable them to attach to selectins on vascular endothelium, thereby promoting their spread to secondary sites.

  • Isoforms & Post-Translational Modification

    CD68 has 3 isomers: P34810-1: 37408 Da (predicted); P34810-2: 31738 Da (predicted); P34810-3: 34654 Da (predicted).
    N- and O-glycosylated

  • SwissProt ID

    P34810

  • Gene ID
    968 [NCBI]
  • Synonyms

    CD 68 antibody; CD68 antibody; CD68 antigen antibody; CD68 molecule antibody; CD68_HUMAN antibody; DKFZp686M18236 antibody; gp11 antibody; Gp110 antibody; LAMP4 antibody; Macrophage antigen CD68 (microsialin) antibody; MACROPHAGE ANTIGEN CD68 antibody; Macrosialin antibody; SCARD1 antibody; Scavenger receptor class D member 1 antibody

  • Research Field

    Immunology

CD68 Antibody (YA798) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 1) and HepG2 (lane 2) and RAW264.7 (lane 3) using CD68 Antibody (YA798) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human non-hodgkin lymphoma using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human head and neck cancer using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 THP-1 cells labeling CD68 Antibody (HY-P80066, red). Cells were stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 THP-1 cells labeling CD68 Antibody (HY-P80066, red). Cells were stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD68 Antibody (YA798) Product Image
    1/16
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • CD68 Antibody (YA798) Product Image
  • Experimental Validation Results for CD68 Antibody (YA798)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 1) and HepG2 (lane 2) and RAW264.7 (lane 3) using CD68 Antibody (YA798) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for CD68 Antibody (YA798)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD68 Antibody (YA798)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human non-hodgkin lymphoma using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD68 Antibody (YA798)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human head and neck cancer using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD68 Antibody (YA798)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD68 Antibody (YA798)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 THP-1 cells labeling CD68 Antibody (HY-P80066, red). Cells were stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD68 Antibody (YA798)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 THP-1 cells labeling CD68 Antibody (HY-P80066, red). Cells were stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • Experimental Validation Results for CD68 Antibody (YA798)
  • CD68 Antibody (YA798) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from THP-1 (lane 1) and HepG2 (lane 2) and RAW264.7 (lane 3) using CD68 Antibody (YA798) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • CD68 Antibody (YA798) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD68 Antibody (YA798) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human non-hodgkin lymphoma using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD68 Antibody (YA798) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human head and neck cancer using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD68 Antibody (YA798) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80066, 1/1000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD68 Antibody (YA798) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 THP-1 cells labeling CD68 Antibody (HY-P80066, red). Cells were stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD68 Antibody (YA798) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 THP-1 cells labeling CD68 Antibody (HY-P80066, red). Cells were stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD68 Antibody (YA798) Product Image WB
  • CD68 Antibody (YA798) Product Image IHC-P
  • CD68 Antibody (YA798) Product Image IHC-P
  • CD68 Antibody (YA798) Product Image IHC-P
  • CD68 Antibody (YA798) Product Image IHC-P
  • CD68 Antibody (YA798) Product Image FC
  • CD68 Antibody (YA798) Product Image FC