IKK gamma Antibody (YA7212)
(Synonyms: FIP3, NEMO, IKBKG, NF-kappa-B essential modulator, FIP-3, IkB kinase-associated protein 1, Inhibitor of nuclear factor kappa-B kinase subunit gamma, NF-kappa-B essential modifier, IKKAP1, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma)
Based on 1 Customer Validation
IKK gamma Antibody (YA7212) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IKK gamma.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IHC-P, IHC-F, IF-Tissue, FC
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in 0.01M tris buffered saline (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
IHC-F
IHC-F: Immunohistochemistry-Frozen
|
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
|
FC
FC: Flow Cytometry
|
|---|---|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-500 | 1:100-500 | 1:100-500 | 1:100-200 |
Product Details
IKK gamma Antibody (YA7212) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IKK gamma.
-
Host Rabbit
-
Clonality Recombinant,Monoclonal
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 48 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 48 kDa
Recombinant human IKK gamma protein
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in 0.01M tris buffered saline (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
IKK gamma is a Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin plays a key role in IKK activation by multiple signaling receptor pathways. Can recognize and bind both 'Lys-63'-linked and linear polyubiquitin upon cell stimulation, with a much higher affinity for linear polyubiquitin. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination |(Microbial infection) Also considered to be a mediator for HTLV-1 Tax oncoprotein activation of NF-kappa-B[1][2][3][4][5][6][7][8][9][10][11][12].
-
Subcellular Localization
Cytoplasm,Nucleus
-
Expression
Tissue_Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas -
Isoforms & Post-Translational Modification
Q9Y6K9 has three isomers: Q9Y6K9-1: 48198 Da (predicted); Q9Y6K9-2: 55787 Da (predicted); Q9Y6K9-3: 36953 Da (predicted).
Phosphorylation at Ser-68 attenuates aminoterminal homodimerization丨Polyubiquitinated on Lys-285 via 'Lys-63'-linked ubiquitin; the ubiquitination is mediated downstream of NOD2 and RIPK2 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway (PubMed:15620648, PubMed:17562858, PubMed:19136968)丨Sumoylated on Lys-277 and Lys-309 with SUMO1; the modification results in phosphorylation of Ser-85 by ATM leading to a replacement of the sumoylation by mono-ubiquitination on these residues丨Neddylated by TRIM40, resulting in stabilization of NFKBIA and down-regulation of NF-kappa-B activity丨(Microbial infection) Cleaved by hepatitis A virus (HAV) protease 3C allowing the virus to disrupt the host innate immune signaling丨(Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1 on both 'Lys-48' and 'Lys-63'-linked ubiquitin chains; leading to NF-kappa-B signaling inhibition丨(Microbial infection) Polyubiquitinated on Lys-309 and Lys-321 via 'Lys-27'-linked ubiquitin by Shigella flexneri E3 ubiquitin-protein ligase ipah9丨(Microbial infection) Polyubiquitination through 'Lys-63' is interrupted by interaction with SARS coronavirus-2/SARS-CoV-2 virus protein ORF9B which inhibits the NF-kappa-B pathway -
Subunit
Homodimer; disulfide-linked (PubMed:18164680)
-
SwissProt ID
-
Synonyms
FIP3, NEMO, IKBKG, NF-kappa-B essential modulator, FIP-3, IkB kinase-associated protein 1, Inhibitor of nuclear factor kappa-B kinase subunit gamma, NF-kappa-B essential modifier, IKKAP1, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma
Documentation
Powered by Bioz
