Copy Link Print Information

Myosin Heavy Chain Smooth Muscle Antibody (YA1620)

(Synonyms: AAT4; FAA4; MYH11; Myosin 11; Myosin heavy chain 11; Myosin heavy chain smooth muscle isoform; Myosin heavy polypeptide 11 smooth muscle; SMHC; SMMHC)
Customer Review

Based on 1 Customer Validation

Myosin Heavy Chain Smooth Muscle Antibody (YA1620) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin Heavy Chain Smooth Muscle.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-1:2000 1:50-1:100

Product Details

Description

Myosin Heavy Chain Smooth Muscle Antibody (YA1620) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin Heavy Chain Smooth Muscle.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 250 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 227 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Myosin, smooth muscle heavy chain 1 and 2 aa2-34.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103973

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Western blot analysis was performed on extracts from Mouse lung (lane 1, 15 μg) using smooth muscle Myosin heavy chain 11 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Immunohistochemical analysis of paraffin-embedded human Testis tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Immunohistochemical analysis of paraffin-embedded human placenta‌ tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    Myosin Heavy Chain Smooth Muscle is a Muscle contraction

  • Subcellular Localization

    Melanosome

  • Expression


    Tissue_specificity:Smooth muscle; expressed in the umbilical artery, bladder, esophagus, and trachea. Isotype 1 is mainly found in slowly contracting tonifying muscles.

  • Isoforms & Post-Translational Modification

    P35749 has 4 isomers: P35749-1: 227339 Da (predicted); P35749-2: 228090 Da (predicted); P35749-3: 224328 Da (predicted); P35749-4: 223577 Da (predicted).

  • Subunit

    Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2)

  • SwissProt ID

    P35749

  • Gene ID
    4629 [NCBI]
  • Synonyms

    AAT4; FAA4; MYH11; Myosin 11; Myosin heavy chain 11; Myosin heavy chain smooth muscle isoform; Myosin heavy polypeptide 11 smooth muscle; SMHC; SMMHC

  • Research Field

    Signal Transduction

Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Mouse lung (lane 1, 15 μg) using smooth muscle Myosin heavy chain 11 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta‌ tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    1/16
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Mouse lung (lane 1, 15 μg) using smooth muscle Myosin heavy chain 11 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta‌ tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Experimental Validation Results for Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Mouse lung (lane 1, 15 μg) using smooth muscle Myosin heavy chain 11 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Testis tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta‌ tissue using Myosin Heavy Chain Smooth Muscle antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81875,1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Myosin Heavy Chain Smooth Muscle antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81875, 1:300 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image WB
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image IHC-P
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image IHC-P
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image IHC-P
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image IHC-P
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image IHC-P
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image IHC-P
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image mIHC
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image mIHC
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image mIHC
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image mIHC
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image mIHC
  • Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Product Image mIHC