STK3/MST-2Antibody (YA2083)
(Synonyms: STK3; Mess1; MST-2; MST2; Serine/threonine kinase 3; KRS1; STE20-like kinase MST2)STK3/MST-2Antibody (YA2083) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STK3/MST-2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP
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Reactivity :
Human, Mouse, Rat, Hamster
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:20 |
Product Details
STK3/MST-2Antibody (YA2083) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STK3/MST-2.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman, Mouse, Rat, Hamster
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Observed Molecular WeightObserved band size: 56 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 56 kDa
Entrez Gene: 6788 Human ; 56274 Mouse ; 65189 Rat
SwissProt: Q13188 Human ; Q9JI10 Mouse ; O54748 Rat
OMIM: 605030 Human
A synthetic peptide of human STK3
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
STK3/MST-2 is a Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].
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Subcellular Localization
Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
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Expression
Tissue_specificity:It is expressed at high levels in adult kidney, bone, and placental tissues, and at extremely low levels in adult heart, lung, and brain tissues.
Induction:Activity increases during mitosis -
Isoforms & Post-Translational Modification
Q13188 has 2 isomers: Q13188-1: 56301 Da (predicted); Q13188-2: 59462 Da (predicted).
Autophosphorylated on two residues Thr-174 and Thr-180, leading to activation (PubMed:23972470, PubMed:29063833). Phosphorylation at Thr-117 and Thr-384 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF1. Phosphorylated at Ser-15 by PLK1, leading to activation (PubMed:21723128). When autophosphorylated at Thr-180, recruits STRIPAK complex and promotes PP2A-mediated dephosphorylation and inactivation of STK3 (PubMed:29063833, PubMed:30622739);Proteolytically cleaved by caspase-3 during apoptosis. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N);Ubiquitinated by TRIM69; leading to its redistribution to the perinuclear cytoskeleton, where it is phosphorylated by PLK1 and subsequently activated -
Subunit
Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation (By similarity). Interacts with and stabilizes SAV1 (PubMed:15688006, PubMed:16930133, PubMed:28087714, PubMed:29063833). Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1 only in the presence of SAV1. Interacts with PKB/AKT1. Forms a tripartite complex with MOBKL1B and STK38. Interacts with RASSF2 (via SARAH domain). Interacts with DLG5 (via PDZ domain 3) (PubMed:28087714). Interacts with LATS1; this interaction is inhibited in the presence of DLG5 (PubMed:28087714). Interacts with MARK3 in the presence of DLG5 (PubMed:28087714). Interacts with RASSF5; this interaction inhibits STK3 autoactivation through heterodimerization (PubMed:23972470). Interacts (when phosphorylated) with SLMAP (via FHA domain); the interaction associates STK3 with the STRIPAK complex (PubMed:29063833, PubMed:30622739)
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SwissProt ID
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Synonyms
STK3; Mess1; MST-2; MST2; Serine/threonine kinase 3; KRS1; STE20-like kinase MST2
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Research Field
Cell Biology
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