ASC1 Antibody (YA6465)

Cat. No.: HY-P86772: Data Sheet COA
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ASC1 Antibody (YA6465) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ASC1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

ASC1 Antibody (YA6465) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ASC1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 66 kDa;

Observed band size: 66 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

Q15650

Gene ID

9325 [NCBI]

Immunogen

Recombinant protein within human ASC1 aa 1-581 / 581.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、Rat brain (lane 3(20μg)) and Rat testis (lane 4(20μg)) using ASC1 Antibody (HY-P86772) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Transcription coactivator which associates with nuclear receptors, transcriptional coactivators including EP300, CREBBP and NCOA1, and basal transcription factors like TBP and TFIIA to facilitate nuclear receptors-mediated transcription (PubMed:10454579, PubMed:25219498). May thereby play an important role in establishing distinct coactivator complexes under different cellular conditions (PubMed:10454579, PubMed:25219498). Plays a role in thyroid hormone receptor and estrogen receptor transactivation (PubMed:10454579, PubMed:25219498). Also involved in androgen receptor transactivation (By similarity). Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1 (PubMed:12077347). Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B (PubMed:12077347). May play a role in the development of neuromuscular junction (PubMed:26924529). May play a role in late myogenic differentiation (By similarity). Also functions as part of the RQC trigger (RQT) complex that activates the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation (PubMed:32099016, PubMed:32579943, PubMed:36302773)

Subcellular Localization：Nucleus,Cytoplasm, cytosol,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Isoforms & Post-Translational Modification：Q15650: 581 amino acids, molecular weight 66146 Da.

Subunit：Interacts with the thyroid hormone receptor/TR (via the ligand-binding domain); this interaction requires the presence of thyroid hormone (PubMed:10454579)

Synonyms

Activating signal cointegrator 1 antibody; ASC 1 antibody; ASC-1 antibody; HsT17391 antibody; Thyroid hormone receptor interactor 4 antibody; Thyroid receptor interacting protein 4 antibody; Thyroid receptor-interacting protein 4 antibody; TR-interacting protein 4 antibody; TR; IP : 4 antibody; TRIP-4 antibody; Activating signal cointegrator 1 antibody; ASC 1 antibody; ASC-1 antibody; HsT17391 antibody; Thyroid hormone receptor interactor 4 antibody; Thyroid receptor interacting protein 4 antibody; Thyroid receptor-interacting protein 4 antibody; TR-interacting protein 4 antibody; TR; IP : 4 antibody; TRIP-4 antibody; Trip4 antibody; TRIP4_HUMAN antibody; ZC2HC5 antibody; Zinc finger C2HC5 type antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)