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ASC1 Antibody (YA6465)

(Synonyms: Activating signal cointegrator 1 antibody; ASC 1 antibody; ASC-1 antibody; HsT17391 antibody; Thyroid hormone receptor interactor 4 antibody; Thyroid receptor interacting protein 4 antibody; Thyroid receptor-interacting protein 4 antibody; TR-interacting protein 4 antibody; TR; IP : 4 antibody; TRIP-4 antibody; Activating signal cointegrator 1 antibody; ASC 1 antibody; ASC-1 antibody; HsT17391 antibody; Thyroid hormone receptor interactor 4 antibody; Thyroid receptor interacting protein 4 antibody; Thyroid receptor-interacting protein 4 antibody; TR-interacting protein 4 antibody; TR; IP : 4 antibody; TRIP-4 antibody; Trip4 antibody; TRIP4_HUMAN antibody; ZC2HC5 antibody; Zinc finger C2HC5 type antibody; )
Customer Review

Based on 1 Customer Validation

ASC1 Antibody (YA6465) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ASC1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • ASC1 Antibody (YA6465) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:200

Product Details

Description

ASC1 Antibody (YA6465) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ASC1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 66 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 66 kDa
Immunogen

Recombinant protein within human ASC1 aa 1-581 / 581.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ASC1 Antibody (YA6465)
    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、Rat brain (lane 3(20μg)) and Rat testis (lane 4(20μg)) using ASC1 Antibody (HY-P86772) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    Immunohistochemical analysis of paraffin-embedded human skin tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    Immunohistochemical analysis of paraffin-embedded human tongue tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    Immunohistochemical analysis of paraffin-embedded human appendix tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    ASC1 transcription coactivator which associates with nuclear receptors, transcriptional coactivators including EP300, CREBBP and NCOA1, and basal transcription factors like TBP and TFIIA to facilitate nuclear receptors-mediated transcription. May thereby play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a role in thyroid hormone receptor and estrogen receptor transactivation. Also involved in androgen receptor transactivation. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. May play a role in the development of neuromuscular junction. May play a role in late myogenic differentiation. Also functions as part of the RQC trigger (RQT) complex that activates the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation[1][2][3][4][5][6][7].

  • Subcellular Localization

    Nucleus,Cytoplasm, cytosol,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Isoforms & Post-Translational Modification

    Q15650: 581 amino acids, molecular weight 66146 Da.

  • Subunit

    Interacts with the thyroid hormone receptor/TR (via the ligand-binding domain); this interaction requires the presence of thyroid hormone (PubMed:10454579)

  • SwissProt ID

    Q15650

  • Gene ID
    9325 [NCBI]
  • Synonyms

    Activating signal cointegrator 1 antibody; ASC 1 antibody; ASC-1 antibody; HsT17391 antibody; Thyroid hormone receptor interactor 4 antibody; Thyroid receptor interacting protein 4 antibody; Thyroid receptor-interacting protein 4 antibody; TR-interacting protein 4 antibody; TR; IP : 4 antibody; TRIP-4 antibody; Activating signal cointegrator 1 antibody; ASC 1 antibody; ASC-1 antibody; HsT17391 antibody; Thyroid hormone receptor interactor 4 antibody; Thyroid receptor interacting protein 4 antibody; Thyroid receptor-interacting protein 4 antibody; TR-interacting protein 4 antibody; TR; IP : 4 antibody; TRIP-4 antibody; Trip4 antibody; TRIP4_HUMAN antibody; ZC2HC5 antibody; Zinc finger C2HC5 type antibody;

ASC1 Antibody (YA6465) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、Rat brain (lane 3(20μg)) and Rat testis (lane 4(20μg)) using ASC1 Antibody (HY-P86772) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skin tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tongue tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human appendix tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ASC1 Antibody (YA6465) Product Image
    1/16
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • ASC1 Antibody (YA6465) Product Image
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、Rat brain (lane 3(20μg)) and Rat testis (lane 4(20μg)) using ASC1 Antibody (HY-P86772) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skin tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tongue tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human appendix tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • Experimental Validation Results for ASC1 Antibody (YA6465)
  • ASC1 Antibody (YA6465) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、Rat brain (lane 3(20μg)) and Rat testis (lane 4(20μg)) using ASC1 Antibody (HY-P86772) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ASC1 Antibody (YA6465) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human skin tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ASC1 Antibody (YA6465) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ASC1 Antibody (YA6465) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tongue tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ASC1 Antibody (YA6465) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human appendix tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ASC1 Antibody (YA6465) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ASC1 Antibody (YA6465) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ASC1 Antibody (YA6465) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using ASC1 Antibody (HY-P86772, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ASC1 Antibody (YA6465) Product Image WB
  • ASC1 Antibody (YA6465) Product Image IHC-P
  • ASC1 Antibody (YA6465) Product Image IHC-P
  • ASC1 Antibody (YA6465) Product Image IHC-P
  • ASC1 Antibody (YA6465) Product Image IHC-P
  • ASC1 Antibody (YA6465) Product Image IHC-P
  • ASC1 Antibody (YA6465) Product Image IHC-P
  • ASC1 Antibody (YA6465) Product Image IHC-P