ARL8B Antibody
(Synonyms: ARL10C, GIE1, ARL8B, ADP-ribosylation factor-like protein 8B, ADP-ribosylation factor-like protein 10C, Novel small G protein indispensable for equal chromosome segregation 1)ARL8B Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ARL8B.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
ARL8B Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ARL8B.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 21 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 18; 21 kDa
Purified recombinant protein of human ARL8B.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ARL8B is a Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of lysosomal positioning which is important for nutrient sensing, natural killer cell-mediated cytotoxicity and antigen presentation. Along with its effectors, orchestrates lysosomal transport and fusion. Localizes specifically to lysosomal membranes and mediates anterograde lysosomal motility by recruiting PLEKHM2, which in turn recruits the motor protein kinesin-1 on lysosomes. Required for lysosomal and cytolytic granule exocytosis. Critical factor involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells. In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function (By similarity). Also acts as a regulator of endosome to lysosome trafficking pathways of special significance for host defense. Recruits RUFY1 onto early endosomes regulating endosomes to trans-Golgi network proteins retrieval. Regulates cargo trafficking to lysosomes by binding to PLEKHM1 and recruiting the HOPS subunit VPS41, resulting in functional assembly of the HOPS complex on lysosomal membranes. Plays an important role in cargo delivery to lysosomes for antigen presentation and microbial killing. Directs the intersection of CD1d with lipid antigens in lysosomes, and plays a role in intersecting phagosomes with lysosomes to generate phagolysosomes that kill microbes. Involved in the process of MHC II presentation. Regulates the delivery of antigens to lysosomes and the formation of MHC II-peptide complexes through the recruitment of the HOPS complex to lysosomes allowing the fusion of late endosomes to lysosomes (By similarity). May play a role in chromosome segregation[1][2][3][4][5][6][7][8][9][10][11][12].
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Subcellular Localization
Late endosome membrane; Lysosome membrane; Cytoplasm, cytoskeleton, spindle; Cell projection, axon; Synapse; Cytolytic granule membrane; Early endosome membrane
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Expression
Tissue_Specificity: Ubiquitously expressed. -
Isoforms & Post-Translational Modification
ARL8B has 2 isoforms, Q9NVJ2-1: amino acid length is 186, molecular weight is 21539 Da (predicted); Q9NVJ2-2: amino acid length is 163, molecular weight is 18642 Da (predicted).Ubiquitinated at Lys-141 by RNF167, leading to its degradation
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Subunit
Interacts with tubulin.
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SwissProt ID
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Synonyms
ARL10C, GIE1, ARL8B, ADP-ribosylation factor-like protein 8B, ADP-ribosylation factor-like protein 10C, Novel small G protein indispensable for equal chromosome segregation 1
Documentation
[1]. Okai T, et al. Novel small GTPase subfamily capable of associating with tubulin is required for chromosome segregation. J Cell Sci. 2004 Sep 15;117(Pt 20):4705-15. [Content Brief]
[2]. Hofmann I, et al. An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility. J Cell Sci. 2006 Apr 15;119(Pt 8):1494-503. [Content Brief]
[3]. Bagshaw RD, et al. The Arf-family protein, Arl8b, is involved in the spatial distribution of lysosomes. Biochem Biophys Res Commun. 2006 Jun 16;344(4):1186-91. [Content Brief]
[4]. Pu J, et al. BORC, a multisubunit complex that regulates lysosome positioning. Dev Cell. 2015 Apr 20;33(2):176-88. [Content Brief]
[5]. Deshar R, et al. RNF167 targets Arl8B for degradation to regulate lysosome positioning and endocytic trafficking. FEBS J. 2016 Dec;283(24):4583-4599. [Content Brief]
[6]. Marwaha R, et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol. 2017 Apr 3;216(4):1051-1070. [Content Brief]
[7]. Rosa-Ferreira C, et al. Arl8 and SKIP act together to link lysosomes to kinesin-1. Dev Cell. 2011 Dec 13;21(6):1171-8. [Content Brief]
[8]. Tuli A, et al. Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer cell-mediated cytotoxicity. Mol Biol Cell. 2013 Dec;24(23):3721-35. [Content Brief]
[9]. Michelet X, et al. Lysosome-Mediated Plasma Membrane Repair Is Dependent on the Small GTPase Arl8b and Determines Cell Death Type in Mycobacterium tuberculosis Infection. J Immunol. 2018 May 1;200(9):3160-3169. [Content Brief]
[10]. Garg S, et al. Lysosomal trafficking, antigen presentation, and microbial killing are controlled by the Arf-like GTPase Arl8b. Immunity. 2011 Aug 26;35(2):182-93. [Content Brief]
[11]. Rawat S, et al. RUFY1 binds Arl8b and mediates endosome-to-TGN CI-M6PR retrieval for cargo sorting to lysosomes. J Cell Biol. 2023 Jan 2;222(1):. [Content Brief]
[12]. Khatter D, et al. The small GTPase Arl8b regulates assembly of the mammalian HOPS complex on lysosomes. J Cell Sci. 2015 May 1;128(9):1746-61. [Content Brief]
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