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NSE Antibody (YA1004)

(Synonyms: ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE)
Customer Review

Based on 1 Customer Validation

NSE Antibody (YA1004) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NSE.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NSE Antibody (YA1004)
  • NSE Antibody (YA1004) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:10000

Product Details

Description

NSE Antibody (YA1004) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NSE.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Species Reactivity Database
Immunogen

Synthetic peptide derived from human NSE.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NSE Antibody (YA1004)
    Western blot analysis was performed on extracts from U-87MG (lane 1, 15 μg), IMR-32 (lane 2, 15 μg), PC-12 (lane 3, 15 μg), Neuro-2a (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using NSE Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    NSE is a Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival

  • Subcellular Localization

    Cytoplasm; Cell membrane

  • Expression


    Tissue_specificity:α/α homodimers are expressed in embryos and most adult tissues. α/β heterodimers and β/β homodimers are present in skeletal muscle, while α/γ heterodimers and γ/γ homodimers are present in neurons.

    Induction:Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jakob disease; (Microbial infection) Activated by human cytomegalovirus (HCMV) UL38 in order to provide the virus with glycosyl building blocks

  • Isoforms & Post-Translational Modification

    P09104 has 2 isomers: P09104-1: 47269 Da (predicted); P09104-2: 42707 Da (predicted).

  • Subunit

    Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific

  • SwissProt ID

    P09104

  • Gene ID
    2026 [NCBI]
  • Synonyms

    ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE

  • Research Field

    Signal Transduction

NSE Antibody (YA1004) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from U-87MG (lane 1, 15 μg), IMR-32 (lane 2, 15 μg), PC-12 (lane 3, 15 μg), Neuro-2a (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using NSE Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • NSE Antibody (YA1004) Product Image
    1/16
  • Experimental Validation Results for NSE Antibody (YA1004)
  • NSE Antibody (YA1004) Product Image
  • Experimental Validation Results for NSE Antibody (YA1004)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from U-87MG (lane 1, 15 μg), IMR-32 (lane 2, 15 μg), PC-12 (lane 3, 15 μg), Neuro-2a (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using NSE Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for NSE Antibody (YA1004)
  • NSE Antibody (YA1004) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from U-87MG (lane 1, 15 μg), IMR-32 (lane 2, 15 μg), PC-12 (lane 3, 15 μg), Neuro-2a (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using NSE Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • NSE Antibody (YA1004) Product Image WB