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Transcription Factor 21 Antibody (YA2101)

(Synonyms: Capsulin; Epicardin; POD 1; POD1; TCF-21; tcf21)
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Based on 1 Customer Validation

Transcription Factor 21 Antibody (YA2101) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transcription Factor 21.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Transcription Factor 21 Antibody (YA2101)
  • Experimental Validation Results for Transcription Factor 21 Antibody (YA2101)
  • Transcription Factor 21 Antibody (YA2101) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:60 1:50-1:100

Product Details

Description

Transcription Factor 21 Antibody (YA2101) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transcription Factor 21.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 26 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 20 kDa
Species Reactivity Database

Entrez Gene: 6943 Human

SwissProt: O43680 Human

Immunogen

A synthesized peptide derived from human TCF21 aa110-179/179.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104465

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Transcription Factor 21 Antibody (YA2101)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293 (lane 2, 15 μg), 293T (lane 3, 15 μg), HT-29 (lane 4, 15 μg), U2OS (lane 5, 15 μg), and A549 (lane 6, 15 μg) using Transcription Factor 21 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Transcription Factor 21 Antibody (YA2101)
    Western blot analysis of extracts from HEK293T(lane 2(20ug) and HEK293T(lane 3(40ug) using Transcription Factor 21 Antibody (HY-P82356) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Transcription Factor 21 is involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May play a role in the specification or differentiation of one or more subsets of epicardial cell types

  • Subcellular Localization

    Nucleus

  • Subunit

    Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3 and binds the E box (5'-CANNTG-3')

  • SwissProt ID

    O43680

  • Gene ID
    6943 [NCBI]
  • Synonyms

    Capsulin; Epicardin; POD 1; POD1; TCF-21; tcf21

  • Research Field

    -

Transcription Factor 21 Antibody (YA2101) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293 (lane 2, 15 μg), 293T (lane 3, 15 μg), HT-29 (lane 4, 15 μg), U2OS (lane 5, 15 μg), and A549 (lane 6, 15 μg) using Transcription Factor 21 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20ug) and HEK293T(lane 3(40ug) using Transcription Factor 21 Antibody (HY-P82356) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Transcription Factor 21 Antibody (YA2101) Product Image
    1/16
  • Experimental Validation Results for Transcription Factor 21 Antibody (YA2101)
  • Experimental Validation Results for Transcription Factor 21 Antibody (YA2101)
  • Transcription Factor 21 Antibody (YA2101) Product Image
  • Experimental Validation Results for Transcription Factor 21 Antibody (YA2101)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293 (lane 2, 15 μg), 293T (lane 3, 15 μg), HT-29 (lane 4, 15 μg), U2OS (lane 5, 15 μg), and A549 (lane 6, 15 μg) using Transcription Factor 21 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Transcription Factor 21 Antibody (YA2101)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20ug) and HEK293T(lane 3(40ug) using Transcription Factor 21 Antibody (HY-P82356) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Transcription Factor 21 Antibody (YA2101)
  • Experimental Validation Results for Transcription Factor 21 Antibody (YA2101)
  • Transcription Factor 21 Antibody (YA2101) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293 (lane 2, 15 μg), 293T (lane 3, 15 μg), HT-29 (lane 4, 15 μg), U2OS (lane 5, 15 μg), and A549 (lane 6, 15 μg) using Transcription Factor 21 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Transcription Factor 21 Antibody (YA2101) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T(lane 2(20ug) and HEK293T(lane 3(40ug) using Transcription Factor 21 Antibody (HY-P82356) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Transcription Factor 21 Antibody (YA2101) Product Image WB
  • Transcription Factor 21 Antibody (YA2101) Product Image WB