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RSK3 Antibody (YA087)

(Synonyms: RPS6KA2; MAPKAPK1C; RSK3; Ribosomal protein S6 kinase alpha-2; S6K-alpha-2; 90 kDa ribosomal protein S6 kinase 2; p90-RSK 2; p90RSK2; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; Ri)
Customer Review

Based on 1 Customer Validation

RSK3 Antibody (YA087) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RSK3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Hamster

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for RSK3 Antibody (YA087)
  • Experimental Validation Results for RSK3 Antibody (YA087)
  • Experimental Validation Results for RSK3 Antibody (YA087)
  • RSK3 Antibody (YA087) Product Image
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Price
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

RSK3 Antibody (YA087) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RSK3.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Hamster
  • Observed Molecular Weight
    Observed band size: 90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 83 kDa
Species Reactivity Database

Entrez Gene: 6196 Human ;

SwissProt: Q15349 Human ;

Immunogen

Synthetic peptide corresponding to Human RSK3.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102991

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RSK3 Antibody (YA087)
    Western blot analysis of extracts from HEK293 (lane 2(20μg) , Hela (lane 3(20μg) , A431 (lane 4(20μg),using RSK3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RSK3 Antibody (YA087)
    Immunocytochemistry analysis of Hela cells labeling RSK3 with RSK3 Antibody (HY-P80887) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RSK3 Antibody (HY-P80887) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for RSK3 Antibody (YA087)
    Immunocytochemistry analysis of U87 cells labeling RSK3 with RSK3 Antibody (HY-P80887) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RSK3 Antibody (HY-P80887)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate

  • Isoforms & Post-Translational Modification

    Q15349 has 3 isomers: Q15349-1: 83239 Da (predicted); Q15349-2: 85532 Da (predicted); Q15349-3: 84014 Da (predicted).
    Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1 (By similarity);N-terminal myristoylation results in an activated kinase in the absence of added growth factors

  • Subunit

    Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity). Interacts with FBXO5; cooperate to induce the metaphase arrest of early blastomeres; increases and stabilizes interaction of FBXO5 with CDC20 (By similarity)

  • SwissProt ID

    Q15349

  • Gene ID
    6196 [NCBI]
  • Synonyms

    RPS6KA2; MAPKAPK1C; RSK3; Ribosomal protein S6 kinase alpha-2; S6K-alpha-2; 90 kDa ribosomal protein S6 kinase 2; p90-RSK 2; p90RSK2; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; Ri

  • Research Field

    Epigenetics and Nuclear Signaling

RSK3 Antibody (YA087) Related Classifications

100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    RSK3 Antibody (YA087) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg) , Hela (lane 3(20μg) , A431 (lane 4(20μg),using RSK3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    RSK3 Antibody (YA087) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling RSK3 with RSK3 Antibody (HY-P80887) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RSK3 Antibody (HY-P80887) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    RSK3 Antibody (YA087) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunocytochemistry analysis of U87 cells labeling RSK3 with RSK3 Antibody (HY-P80887) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RSK3 Antibody (HY-P80887)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • RSK3 Antibody (YA087) Product Image
    1/16
  • Experimental Validation Results for RSK3 Antibody (YA087)
  • Experimental Validation Results for RSK3 Antibody (YA087)
  • Experimental Validation Results for RSK3 Antibody (YA087)
  • RSK3 Antibody (YA087) Product Image
  • RSK3 Antibody (YA087) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 2(20μg) , Hela (lane 3(20μg) , A431 (lane 4(20μg),using RSK3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • RSK3 Antibody (YA087) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling RSK3 with RSK3 Antibody (HY-P80887) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RSK3 Antibody (HY-P80887) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • RSK3 Antibody (YA087) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U87 cells labeling RSK3 with RSK3 Antibody (HY-P80887) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RSK3 Antibody (HY-P80887)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • RSK3 Antibody (YA087) Product Image WB
  • RSK3 Antibody (YA087) Product Image ICC
  • RSK3 Antibody (YA087) Product Image ICC