Cytokeratin 13 Antibody (YA2695)
(Synonyms: Keratin; type I cytoskeletal 13; Krt1-13; Cytokeratin 13; CK13; Keratin 13; KRT13; WSN2)Based on 1 Customer Validation
Cytokeratin 13 Antibody (YA2695) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 13.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
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| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:200 |
Product Details
Cytokeratin 13 Antibody (YA2695) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 13.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 50 kDaNote: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 50 kDa
A synthesized peptide derived from human Cytokeratin 13 aa121-155/458.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis was performed on extracts from Haca-T (lane 1, 20 μg), and Mouse skin (lane 2, 20 μg) using Cytokeratin 13 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Background
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Function
Cytokeratin 13 is a Type 1 keratin (Probable). Maintains postnatal tongue mucosal cell homeostasis and tissue organization in response to mechanical stress, potentially via regulation of the G1/S phase cyclins CCNE1 and CCNE2
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Expression
Tissue_specificity:It is expressed in certain epidermal sweat gland ducts (protein level) as well as in the external os of the cervix, esophagus, and placenta. -
Isoforms & Post-Translational Modification
P13646 has 3 isomers: P13646-1: 49588 Da (predicted); P13646-2: 38581 Da (predicted); P13646-3: 45867 Da (predicted).
O-glycosylated; glycans consist of single N-acetylglucosamine residues -
Subunit
Heterotetramer of two type I and two type II keratins
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SwissProt ID
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Synonyms
Keratin; type I cytoskeletal 13; Krt1-13; Cytokeratin 13; CK13; Keratin 13; KRT13; WSN2
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Research Field
Tags & Cell Markers
Documentation
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Data Sheet (232 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)