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TR4 Antibody (YA6889)

(Synonyms: hTAK1 antibody)
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Based on 1 Customer Validation

TR4 Antibody (YA6889) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TR4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TR4 Antibody (YA6889)
  • TR4 Antibody (YA6889) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:200-1:1000

Product Details

Description

TR4 Antibody (YA6889) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TR4.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 65 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size：65 kDa
Immunogen

Recombinant protein within Human TR4 aa 497-596 / 596.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TR4 Antibody (YA6889)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、Jurkat (lane 2(20μg)) 、HEK293 (lane 3(20μg)) and NIH-3T3 (lane 4(20μg)) using TR4 Antibody (HY-P87201) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    TR4 is an Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear receptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior. Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation

  • Subcellular Localization

    Nucleus

  • Expression

    Induction: Induced by oxidative stress via FOXO3 activation

  • Isoforms & Post-Translational Modification

    P49116 has two isomers: P49116-1: 65414 Da (predicted); P49116-2: 67345 Da (predicted).
    Phosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity

  • Subunit

    Homodimer; can bind DNA as homodimer (By similarity)

  • SwissProt ID

    P49116

  • Synonyms

    hTAK1 antibody

TR4 Antibody (YA6889) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、Jurkat (lane 2(20μg)) 、HEK293 (lane 3(20μg)) and NIH-3T3 (lane 4(20μg)) using TR4 Antibody (HY-P87201) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • TR4 Antibody (YA6889) Product Image
    1/16
  • Experimental Validation Results for TR4 Antibody (YA6889)
  • TR4 Antibody (YA6889) Product Image
  • Experimental Validation Results for TR4 Antibody (YA6889)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、Jurkat (lane 2(20μg)) 、HEK293 (lane 3(20μg)) and NIH-3T3 (lane 4(20μg)) using TR4 Antibody (HY-P87201) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TR4 Antibody (YA6889)
  • TR4 Antibody (YA6889) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、Jurkat (lane 2(20μg)) 、HEK293 (lane 3(20μg)) and NIH-3T3 (lane 4(20μg)) using TR4 Antibody (HY-P87201) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • TR4 Antibody (YA6889) Product Image WB