PIP5K1C Antibody (YA6704)

Cat. No.: HY-P87011: Data Sheet User Guide for Antibodies
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PIP5K1C Antibody (YA6704) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PIP5K1C.

For research use only. We do not sell to patients.

Size	Price	Stock
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PIP5K1C Antibody (YA6704) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PIP5K1C.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 73 kDa;

Observed band size: 87/90 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O60331

Gene ID

23396 [NCBI]

Immunogen

Recombinant protein within human PIP5K1C aa 31-470 / 668.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility (PubMed:12422219, PubMed:22942276). PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3) (Probable). PIP5K1A-mediated phosphorylation of PtdIns(4)P is the predominant pathway for PtdIns(4,5)P2 synthesis (By similarity). Together with PIP5K1A, is required for phagocytosis, both enzymes regulating different types of actin remodeling at sequential steps (By similarity). Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport (By similarity). Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis (PubMed:12847086). Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2) (PubMed:12847086). Required for clathrin-coated pits assembly at the synapse (PubMed:17261850). Participates in cell junction assembly (PubMed:17261850). Modulates adherens junctions formation by facilitating CDH1/cadherin trafficking (PubMed:17261850). Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions (PubMed:12422219). Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins (PubMed:12422219). Required for uropodium formation and retraction of the cell rear during directed migration (By similarity). Has a role in growth factor-stimulated directional cell migration and adhesion (By similarity). Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor (PubMed:17635937). Negative regulator of T-cell activation and adhesion (By similarity). Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor (By similarity). Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth (By similarity)

Subcellular Localization：Cell membrane,Endomembrane system,Cytoplasm,Cell junction, focal adhesion,Cell junction, adherens junction,Cell projection, ruffle membrane,Cell projection, phagocytic cup,Cell projection, uropodium,Cytoplasm,Nucleus

Expression：
Tissue_Specificity: Isoform 1 is strongly expressed in brain and also detected in heart and lung|Isoform 2 is strongly expressed in pancreas and liver and in lesser quantities in brain, heart, lung and kidney|Isoform 3 is detected in large amounts in heart and large intestine, is also present in lung, pancreas and thyroid, and to a lesser extent in brain, stomach and kidney

Isoforms & Post-Translational Modification：O60331 has four isomers: O60331-1: 73260 Da (predicted); O60331-2: 76620 Da (predicted); O60331-3: 77484 Da (predicted); O60331-4: 70214 Da (predicted).
Phosphorylation on Ser-650 negatively regulates binding to TLN2 and is strongly stimulated in mitosis丨Acetylation at Lys-265 and Lys-268 seems to decrease lipid 1-phosphatidylinositol-4-phosphate 5-kinase activity

Subunit：Interacts with TLN1 (By similarity)

Synonyms

Diphosphoinositide kinase antibody; LCCS3 antibody; Phosphatidylinositol-4-phosphate 5-kinase type I gamma antibody; Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma antibody; PI51C antibody; PI51C_HUMAN antibody; PIP5K GAMMA antibody; PIP5K1-gamma antibody; Pip5k1c antibody; PIP5KIgamma antibody; Diphosphoinositide kinase antibody; LCCS3 antibody; Phosphatidylinositol-4-phosphate 5-kinase type I gamma antibody; Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma antibody; PI51C antibody; PI51C_HUMAN antibody; PIP5K GAMMA antibody; PIP5K1-gamma antibody; Pip5k1c antibody; PIP5KIgamma antibody; PtdIns(4)P 5 kinase gamma antibody; PtdIns(4)P-5-kinase 1 gamma antibody; Type I P; IP : kinase antibody;

Documentation

User Guide for Antibodies (1077 KB)