Aquaporin 2 Antibody (YA3999)(PBS only)

Cat. No.: HY-P84302A: Data Sheet User Guide for Antibodies
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Aquaporin 2 Antibody (YA3999) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Aquaporin 2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Aquaporin 2 Antibody (YA3999) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Aquaporin 2.

Host

Mouse

Molecular Weight

Predicted band size: 29 kDa;

Observed band size: 40-50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P41181

Gene ID

359 [NCBI]

Immunogen

Purified recombinant fragment of human AQP2 (AA: 149-271) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed:15509592, PubMed:7510718, PubMed:7524315, PubMed:8140421, PubMed:8584435). Plays an essential role in renal water homeostasis (PubMed:15509592, PubMed:7524315, PubMed:8140421). Could also be permeable to glycerol (PubMed:8584435)

Subcellular Localization：Apical cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein; Cytoplasmic vesicle membrane; Multi-pass membrane protein; Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:It is expressed (protein level) in the renal medullary collecting ducts (PubMed: 7510718) . It was detected in the kidneys (PubMed: 7510718) .

Subunit：Homotetramer (PubMed:24733887). Interacts with micropeptide MIAC; the interaction leads to a reduction of filamentous actin fibers and inhibition of the EREG/EGFR signaling pathway (PubMed:32176498, PubMed:36117171)

Synonyms

AQP-CD; WCH-CD

Documentation

User Guide for Antibodies (1077 KB)