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PR3 Antibody (YA2756)

(Synonyms: PRTN3; ACPA; CANCA)
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Based on 1 Customer Validation

PR3 Antibody (YA2756) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PR3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PR3 Antibody (YA2756)
  • PR3 Antibody (YA2756) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

PR3 Antibody (YA2756) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PR3.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Species Reactivity Database

Entrez Gene: 5657 Human

SwissProt: P24158 Human

Immunogen

A synthesized peptide derived from human PR3 aa64-100.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105129

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PR3 Antibody (YA2756)
    Western blot analysis of extracts from U937(lane 2(20μg) and U937(lane 3(40μg) using PR3 (HY-P83011) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    PR3 serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. Plays a role in neutrophil transendothelial migration, probably when associated with CD177. Triggers inflammatory processes in neutrophils by interacting with ADGRG3 upstream of F2RL1/PAR2 activation[1][2][3][4][5][6].

  • Subcellular Localization

    Cytoplasmic granule; Secreted; Cell membrane; Peripheral membrane protein; Extracellular side; Membrane raft; Peripheral membrane protein; Extracellular side

  • Expression


    Tissue_specificity:Expression at protein levels in polymorphonuclear leukocytes (PubMed:2033050, PubMed:3198760, PubMed:7897245) . Expression at protein levels in neutrophils (PubMed:17244676, PubMed:18462208, PubMed:21193407, PubMed:22266279, PubMed:28240246) . Expression in differentiating neutrophils (PubMed:18462208) .

    Induction:Induced during CSF3/G-CSF-mediated neutrophil differentiation

  • Subunit

    May form dimers (PubMed:28240246). Interacts with CD177; the interaction tethers PRTN3 to the cell surface; the interaction is direct (PubMed:17244676, PubMed:28240246). Interacts with SERPINB1 (PubMed:30692621). Interacts with ADGRG3 (PubMed:36302784)

  • SwissProt ID

    P24158

  • Gene ID
    5657 [NCBI]
  • Synonyms

    PRTN3; ACPA; CANCA

  • Research Field

    Signal Transduction

PR3 Antibody (YA2756) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U937(lane 2(20μg) and U937(lane 3(40μg) using PR3 (HY-P83011) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PR3 Antibody (YA2756) Product Image
    1/16
  • Experimental Validation Results for PR3 Antibody (YA2756)
  • PR3 Antibody (YA2756) Product Image
  • Experimental Validation Results for PR3 Antibody (YA2756)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U937(lane 2(20μg) and U937(lane 3(40μg) using PR3 (HY-P83011) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PR3 Antibody (YA2756)
  • PR3 Antibody (YA2756) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U937(lane 2(20μg) and U937(lane 3(40μg) using PR3 (HY-P83011) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PR3 Antibody (YA2756) Product Image WB