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Adenosine A1 Receptor Antibody (YA3341)

(Synonyms: ADORA1; Adenosine receptor A1)
Customer Review

Based on 1 Customer Validation

Adenosine A1 Receptor Antibody (YA3341) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Adenosine A1 Receptor.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
  • Adenosine A1 Receptor Antibody (YA3341) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

Adenosine A1 Receptor Antibody (YA3341) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Adenosine A1 Receptor.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 37 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 37 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human Adenosine A1 Receptor

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
    Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), 3T3 (lane 4(20μg) and Hela (lane 5(20μg) using Adenosine A1 Receptor Antibody (HY-P83596) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
    Immunocytochemistry analysis of MCF-7 cells labeling Adenosine A1 Receptor Antibody(HY-P83596) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adenosine A1 Receptor Antibody(HY-P83596) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
    Immunocytochemistry analysis of MCF-7 cells labeling Adenosine A1 Receptor Antibody(HY-P83596) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adenosine A1 Receptor Antibody(HY-P83596) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Adenosine A1 Receptor receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Isoforms & Post-Translational Modification

    P30542 has 2 isomers: P30542-1: 36512 Da (predicted); P30542-2: 13348 Da (predicted).

  • SwissProt ID

    P30542

  • Gene ID
    134 [NCBI]
  • Synonyms

    ADORA1; Adenosine receptor A1

  • Research Field

    Cell Biology

Adenosine A1 Receptor Antibody (YA3341) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), 3T3 (lane 4(20μg) and Hela (lane 5(20μg) using Adenosine A1 Receptor Antibody (HY-P83596) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Adenosine A1 Receptor Antibody(HY-P83596) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adenosine A1 Receptor Antibody(HY-P83596) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Adenosine A1 Receptor Antibody(HY-P83596) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adenosine A1 Receptor Antibody(HY-P83596) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Adenosine A1 Receptor Antibody (YA3341) Product Image
    1/16
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
  • Adenosine A1 Receptor Antibody (YA3341) Product Image
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), 3T3 (lane 4(20μg) and Hela (lane 5(20μg) using Adenosine A1 Receptor Antibody (HY-P83596) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Adenosine A1 Receptor Antibody(HY-P83596) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adenosine A1 Receptor Antibody(HY-P83596) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Adenosine A1 Receptor Antibody(HY-P83596) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adenosine A1 Receptor Antibody(HY-P83596) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
  • Experimental Validation Results for Adenosine A1 Receptor Antibody (YA3341)
  • Adenosine A1 Receptor Antibody (YA3341) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), 3T3 (lane 4(20μg) and Hela (lane 5(20μg) using Adenosine A1 Receptor Antibody (HY-P83596) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Adenosine A1 Receptor Antibody (YA3341) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Adenosine A1 Receptor Antibody(HY-P83596) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adenosine A1 Receptor Antibody(HY-P83596) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Adenosine A1 Receptor Antibody (YA3341) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Adenosine A1 Receptor Antibody(HY-P83596) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adenosine A1 Receptor Antibody(HY-P83596) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Adenosine A1 Receptor Antibody (YA3341) Product Image WB
  • Adenosine A1 Receptor Antibody (YA3341) Product Image ICC
  • Adenosine A1 Receptor Antibody (YA3341) Product Image ICC