Adenosine A1 Receptor Antibody (YA3341)

Cat. No.: HY-P83596: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Adenosine A1 Receptor Antibody (YA3341) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Adenosine A1 Receptor.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈	재고	수량
무료 샘플	Apply now
10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Adenosine A1 Receptor Antibody (YA3341) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
제품 설명

제품 설명

Adenosine A1 Receptor Antibody (YA3341) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Adenosine A1 Receptor.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 37 kDa;

Observed band size: 37 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P30542

Gene ID

134 [NCBI]

Immunogen

A synthetic peptide of human Adenosine A1 Receptor

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), 3T3 (lane 4(20μg) and Hela (lane 5(20μg) using Adenosine A1 Receptor Antibody (HY-P83596) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of MCF-7 cells labeling Adenosine A1 Receptor Antibody(HY-P83596) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adenosine A1 Receptor Antibody(HY-P83596) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of MCF-7 cells labeling Adenosine A1 Receptor Antibody(HY-P83596) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adenosine A1 Receptor Antibody(HY-P83596) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase

Subcellular Localization：Cell membrane; Multi-pass membrane protein

Isoforms & Post-Translational Modification：P30542 has 2 isomers: P30542-1: 36512 Da (predicted); P30542-2: 13348 Da (predicted).

Database

Entrez Gene: 134 Human ; 11539 Mouse ; 29290 Rat

SwissProt: P30542 Human ; Q60612 Mouse ; P25099 Rat

OMIM: 102775 Human

Research Field

Cell Biology

Synonyms

ADORA1; Adenosine receptor A1

각종 서류

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Adenosine A1 Receptor Antibody (YA3341) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

최근 본 상품:

상품명

Requested Quantity *

고객명 *

호칭

메일주소 *

전화번호 *

Department

회사명 *

City

도

Country or Region *

비고

상품명

Lot #

고객명 *

Country or Region *

메일주소 *

비고

Name
이메일 *

	Sorry, but the email address you supplied was invalid.

Adenosine A1 Receptor Antibody (YA3341)

Adenosine A1 Receptor Antibody (YA3341) Featured Recommendations:

Product Detail

Verification Image

Background

제품 설명

Adenosine A1 Receptor Antibody (YA3341) Related Classifications

최근 본 상품:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report