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SSR1 (TRAP alpha) Antibody (YA7781)

(Synonyms: TRAPA)
Customer Review

Based on 1 Customer Validation

SSR1 (TRAP alpha) Antibody (YA7781) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SSR1 (TRAP alpha).

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
  • SSR1 (TRAP alpha) Antibody (YA7781) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-2000 1:150-500 1:50-100 1:100

Product Details

Description

SSR1 (TRAP alpha) Antibody (YA7781) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SSR1 (TRAP alpha).

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 32.1 kDa
Immunogen

Full length human recombinant protein of human SSR1 produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
    Western blot analysis of extracts from A431 (lane2(20μg), HepG2 (lane3(20μg), Mouse liver tissue (lane4(20μg) and Rat liver tissue (lane5(20μg) using SSR1 (TRAP alpha) Antibody (HY-P88097). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
    Immunocytochemistry analysis of Hela cells labeling SSR1 (TRAP alpha) with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
    Immunocytochemistry analysis of PC-3 cells labeling SSR1 (TRAP alpha) with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    SSR1 is a TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins

  • Subcellular Localization

    Endoplasmic reticulum membrane

  • Isoforms & Post-Translational Modification

    P43307 has two isomers: P43307-1: 32235 Da (predicted); P43307-2: 29373 Da (predicted).

  • Subunit

    Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma (PubMed:36697828)

  • SwissProt ID

    P43307

  • Synonyms

    TRAPA

SSR1 (TRAP alpha) Antibody (YA7781) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane2(20μg), HepG2 (lane3(20μg), Mouse liver tissue (lane4(20μg) and Rat liver tissue (lane5(20μg) using SSR1 (TRAP alpha) Antibody (HY-P88097). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling SSR1 (TRAP alpha) with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling SSR1 (TRAP alpha) with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SSR1 (TRAP alpha) Antibody (YA7781) Product Image
    1/16
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
  • SSR1 (TRAP alpha) Antibody (YA7781) Product Image
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane2(20μg), HepG2 (lane3(20μg), Mouse liver tissue (lane4(20μg) and Rat liver tissue (lane5(20μg) using SSR1 (TRAP alpha) Antibody (HY-P88097). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling SSR1 (TRAP alpha) with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling SSR1 (TRAP alpha) with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
  • Experimental Validation Results for SSR1 (TRAP alpha) Antibody (YA7781)
  • SSR1 (TRAP alpha) Antibody (YA7781) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431 (lane2(20μg), HepG2 (lane3(20μg), Mouse liver tissue (lane4(20μg) and Rat liver tissue (lane5(20μg) using SSR1 (TRAP alpha) Antibody (HY-P88097). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • SSR1 (TRAP alpha) Antibody (YA7781) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling SSR1 (TRAP alpha) with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SSR1 (TRAP alpha) Antibody (YA7781) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling SSR1 (TRAP alpha) with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SSR1 (TRAP alpha) Antibody (HY-P88097) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SSR1 (TRAP alpha) Antibody (YA7781) Product Image WB
  • SSR1 (TRAP alpha) Antibody (YA7781) Product Image ICC
  • SSR1 (TRAP alpha) Antibody (YA7781) Product Image ICC