ATF6 Antibody (YA6318)
(Synonyms: Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6-alpha, ATF6)ATF6 Antibody (YA6318) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATF6.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-5000 | 1:200-1000 | 1:5000-20000 | 1:50-200 |
Product Details
ATF6 Antibody (YA6318) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATF6.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 100 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 75 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ATF6 (Activating transcription factor 6) is an endoplasmic reticulum (ER) -localised protein and member of the leucine zipper family of transcription factors. ATF6 plays an important role in transducing signals linked to the ER stress. In the absence of ER stress, GRP78 binds to the ER luminal domain of ATF6 and anchors ATF6 in the ER. Upon ER stress, GRP78 dissociates from, and thus, releases ATF6, allowing it to relocate to the next destination in the secretory pathway, the Golgi. In the Golgi, ATF6 is cleaved by S1P and S2P proteases by regulated intramembrane proteolysis. This cleavage liberates the N-terminal 50 kD cytosolic portion of ATF6 (activated form), which has a nuclear localization sequence, facilitating its movement to the nucleus, where it acts as a transcription factor to induce genes that encode stress-reducing chaperones and foldases that slow the pace of protein translation.
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Subcellular Localization
Endoplasmic reticulum membrane; Single-pass type II membrane protein; Golgi apparatus membrane; Single-pass type II membrane protein; Nucleus
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Expression
Tissue_specificity:general expression -
Subunit
Interacts with XBP1 isoform 2; the interaction occurs in a ER stress-dependent manner (PubMed:17765680). Interacts with LACC1 (PubMed:31875558)
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SwissProt ID
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Synonyms
Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6-alpha, ATF6
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