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IGFBP2 Antibody (YA9583)

(Synonyms: mIGFBP-2, Insulin-like growth factor-binding protein 2, IGFBP-2, IGF-binding protein 2, IBP-2)
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Based on 1 Customer Validation

IGFBP2 Antibody (YA9583) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclin950.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for IGFBP2 Antibody (YA9583)
  • IGFBP2 Antibody (YA9583) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:5000 1:100 1:500 1:50-200 1:1000 1-2μg per 100-500μg Total protein

Product Details

Description

IGFBP2 Antibody (YA9583) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP2.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Immunogen

A synthesized Recombinant protein derived from mouse IGFBP2 aa 1-305.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclin950.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at 2-8°C for 1 year, do not freeze.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IGFBP2 Antibody (YA9583)
    Western blot analysis was performed on protein extracts (35 μg) from MCF-7 (lane 2), A549 (lane 3), and Mouse serum (lane 4) using IGFBP2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:5000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    IGFBP2 is a Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation. Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif. Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells. Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury[1][2].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_Specificity: Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary.

  • Isoforms & Post-Translational Modification

    IGFBP2 has an amino acid length of 305, molecular weight is 32847 Da.
    Cleaved by MMP9 leading to release of free IGF2 from IGFBP2-IGF2 complex, which contributes to enhance the motility and the growth of astrocytes

  • Subunit

    Interacts with IGF1.

  • SwissProt ID

    P47877

  • Gene ID
    16008 [NCBI]
  • Synonyms

    mIGFBP-2, Insulin-like growth factor-binding protein 2, IGFBP-2, IGF-binding protein 2, IBP-2

References

[1]. DeMambro VE, et al. Gender-specific changes in bone turnover and skeletal architecture in igfbp-2-null mice. Endocrinology. 2008 May;149(5):2051-61. [Content Brief]

[2]. Xi G, et al. IRS-1 Functions as a Molecular Scaffold to Coordinate IGF-I/IGFBP-2 Signaling During Osteoblast Differentiation. J Bone Miner Res. 2016 Jun;31(6):1300-14. [Content Brief]

IGFBP2 Antibody (YA9583) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (35 μg) from MCF-7 (lane 2), A549 (lane 3), and Mouse serum (lane 4) using IGFBP2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:5000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • IGFBP2 Antibody (YA9583) Product Image
    1/16
  • Experimental Validation Results for IGFBP2 Antibody (YA9583)
  • IGFBP2 Antibody (YA9583) Product Image
  • Experimental Validation Results for IGFBP2 Antibody (YA9583)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (35 μg) from MCF-7 (lane 2), A549 (lane 3), and Mouse serum (lane 4) using IGFBP2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:5000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for IGFBP2 Antibody (YA9583)
  • IGFBP2 Antibody (YA9583) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (35 μg) from MCF-7 (lane 2), A549 (lane 3), and Mouse serum (lane 4) using IGFBP2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:5000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • IGFBP2 Antibody (YA9583) Product Image WB