MBD1 Antibody (YA2441)
(Synonyms: CXXC 3; Mbd1; MECP1 COMPLEX; PCM1; RFT)MBD1 Antibody (YA2441) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MBD1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP
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Reactivity :
Human
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:200 | 1:20 |
Product Details
MBD1 Antibody (YA2441) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MBD1.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 67 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 67 kDa
A synthesized peptide derived from human MBD1 aa550-605/605.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MBD1 transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters
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Subcellular Localization
Nucleus; Nucleus matrix; Nucleus speckle; Chromosome
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Expression
Tissue_specificity:Widely expressed
Induction:Up-regulated by interferon -
Isoforms & Post-Translational Modification
Q9UIS9 has 11 isomers: Q9UIS9-1: 66607 Da (predicted); Q9UIS9-2: 64677 Da (predicted); Q9UIS9-4: 55167 Da (predicted); Q9UIS9-5: 61265 Da (predicted); Q9UIS9-6: 60848 Da (predicted); Q9UIS9-7: 60001 Da (predicted); Q9UIS9-8: 59158 Da (predicted); Q9UIS9-9: 69617 Da (predicted); Q9UIS9-10: 62639 Da (predicted); Q9UIS9-11: 58752 Da (predicted); Q9UIS9-12: 72126 Da (predicted).
Sumoylated, sumoylation may increase interaction with ATF7IP -
Subunit
Interacts with OASL, ATF7IP, ATF7IP2 and BAHD1 (PubMed:12665582, PubMed:14728690, PubMed:15691849, PubMed:16757475, PubMed:19666599). Binds CHAF1A and the SUV39H1-CBX5 complex via the MBD domain (PubMed:12697822, PubMed:12711603). Binds MGP via the TRD domain (PubMed:14555760). May be part of the MeCP1 complex (PubMed:9207790)
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SwissProt ID
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Synonyms
CXXC 3; Mbd1; MECP1 COMPLEX; PCM1; RFT
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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