CD33 Antibody (YA4780)
(Synonyms: CD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33)CD33 Antibody (YA4780) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD33.
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Host:
Mouse
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Application:
WB, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:10000 |
Product Details
CD33 Antibody (YA4780) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD33.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 40 kDa;
Purified recombinant fragment of CD33 (48-258) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD33 (Siglec-3) is an important member of the sialic acid-binding immunoglobulin-like lectin family, one of the most classic surface markers of myeloid cells, and one of the most important targets in acute myeloid leukemia (AML). CD33 is an inhibitory immune receptor belonging to the immunoglobulin superfamily (IgSF), and its intracellular structure contains the immunoreceptor tyrosine inhibitory motif (ITIM).
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Peroxisome
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Expression
Tissue_specificity:Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells -
Isoforms & Post-Translational Modification
CD33 has 3 isomers: P20138-1: 39825 Da (predicted); P20138-2: 33890 Da (predicted); P20138-3: 25293 Da (predicted).
Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition;Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent -
Subunit
Homodimer; disulfide-linked (PubMed:10887109). Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation (PubMed:10206955, PubMed:10556798).
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SwissProt ID
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Synonyms
CD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33
Documentation
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