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NEK2 Antibody (YA1910)

(Synonyms: HsPK21; NEK2; NEK2A; NLK1)
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Based on 1 Customer Validation

NEK2 Antibody (YA1910) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEK2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NEK2 Antibody (YA1910)
  • NEK2 Antibody (YA1910) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

NEK2 Antibody (YA1910) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEK2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 48,44 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Species Reactivity Database

Entrez Gene: 4751 Human

SwissProt: P51955 Human

Immunogen

A synthesized peptide derived from human NEK2 aa21-47.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104278

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NEK2 Antibody (YA1910)
    Western blot analysis of extracts from Hela (lane 2, 20ug), A549 (lane 3, 20ug), HL-60 (lane 4, 20ug) and K562 (lane 5, 20ug) using NEK2 Antibody (HY-P82165). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    NEK2 (NIMA-related kinase 2) is a serine/threonine kinase that localizes predominantly to the centrosome and functions as a key regulator of centrosome separation, bipolar spindle assembly, chromosome segregation, and mitotic progression[1][2]. NEK2 expression increases during S and G2 phases of the cell cycle, supporting its role in coordinating accurate cell division and maintenance of genomic stability[1][2]. Mechanistically, NEK2 phosphorylates proteins involved in centrosome disjunction and chromosome segregation, and dysregulated NEK2 activity promotes premature centrosome splitting, chromosomal instability, aneuploidy, and aberrant mitotic progression[1][2][3]. In addition to its canonical centrosomal functions, NEK2 can localize to the nucleus, interact with splicing factors, and regulate alternative splicing programs linked to cell survival and apoptosis[2]. In disease contexts, NEK2 is frequently overexpressed in multiple malignancies, where elevated expression is associated with tumor progression, drug resistance, chromosomal instability, and poor clinical prognosis[3][4]. Compared with other members of the NIMA-related kinase family, NEK2 is distinguished by its prominent role in centrosome dynamics and mitotic regulation, and alternative splicing generates isoforms with distinct C-terminal regions that may differ in subcellular localization and regulatory properties[1][2]. For experimental applications, NEK2 has emerged as a therapeutic target, and small-molecule inhibitors have demonstrated the ability to suppress NEK2 kinase activity in cellular and preclinical cancer models, supporting their utility for investigating mitotic regulation and cancer biology[3][5].

  • Subcellular Localization

    Nucleus; Nucleus, nucleolus; Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle pole; Chromosome, centromere, kinetochore; Chromosome, centromere; Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Expression


    Tissue_specificity:Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors

    Induction:Expression and activity peak in the G2 phase of the mitotic cycle and decrease once the cells have entered mitosis due to degradation by the anaphase promoting complex APC/C-CDC20. In G1 phase, both isoform 1 and isoform 2 are almost undetectable. However, at the G1/S transition, there is an increase in expression of both isoforms which then remain at this increased level throughout S and G2. At the onset of mitosis, isoform 1 undergoes a rapid disappearance whereas isoform 2 continues to be present at about the same level as in G2. During the rest of mitosis, isoform 1 remains absent, while isoform 2 only begins to decline upon re-entry into the next G1 phase

  • Isoforms & Post-Translational Modification

    P51955 has 4 isomers: P51955-1: 51763 Da (predicted); P51955-2: 44906 Da (predicted); P51955-3: 37956 Da (predicted); P51955-4: 50909 Da (predicted).
    Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome

  • Subunit

    Isoform 1, isoform 2 and isoform 4 form homo- and heterodimers. Isoform 1 interacts with CDC20, CTNB1, MAD1L1, MAPK, NEK11, NPM1, NDC80, PCNT and SGO1 (PubMed:14978040, PubMed:15161910, PubMed:15358203, PubMed:15388344, PubMed:17621308, PubMed:18086858, PubMed:18297113, PubMed:20034488, PubMed:20599736).

  • SwissProt ID

    P51955

  • Gene ID
    4751 [NCBI]
  • Synonyms

    HsPK21; NEK2; NEK2A; NLK1

  • Research Field

    Cell Biology

[1]. Naro C, et al. The centrosomal kinase NEK2 is a novel splicing factor kinase involved in cell survival. Nucleic Acids Res. 2014 Mar;42(5):3218-27. [Content Brief]

[2]. Xia J, et al. Role of NEK2 in tumorigenesis and tumor progression. Trends Mol Med. 2025 Jan;31(1):79-93. [Content Brief]

NEK2 Antibody (YA1910) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug), A549 (lane 3, 20ug), HL-60 (lane 4, 20ug) and K562 (lane 5, 20ug) using NEK2 Antibody (HY-P82165). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • NEK2 Antibody (YA1910) Product Image
    1/16
  • Experimental Validation Results for NEK2 Antibody (YA1910)
  • NEK2 Antibody (YA1910) Product Image
  • Experimental Validation Results for NEK2 Antibody (YA1910)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug), A549 (lane 3, 20ug), HL-60 (lane 4, 20ug) and K562 (lane 5, 20ug) using NEK2 Antibody (HY-P82165). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NEK2 Antibody (YA1910)
  • NEK2 Antibody (YA1910) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2, 20ug), A549 (lane 3, 20ug), HL-60 (lane 4, 20ug) and K562 (lane 5, 20ug) using NEK2 Antibody (HY-P82165). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • NEK2 Antibody (YA1910) Product Image WB