Phospho-EGFR (Tyr1068) Antibody (YA7493)

Cat. No.: HY-P87808: Data Sheet User Guide for Antibodies
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Phospho-EGFR (Tyr1068) Antibody (YA7493) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-EGFR (Tyr1068).

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Phospho-EGFR (Tyr1068) Antibody (YA7493) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-EGFR (Tyr1068).

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 134 kDa;

Observed band size: 175 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P00533

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Sensitivity	Endogenous	Purity	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG, Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:10805725, PubMed:27153536, PubMed:2790960, PubMed:35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:12297049, PubMed:15611079, PubMed:17909029, PubMed:20837704, PubMed:27153536, PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146).

Subcellular Localization：Cell membrane,Endoplasmic reticulum membrane,Golgi apparatus membrane,Nucleus membrane,Endosome,Endosome membrane,Nucleus,Secreted

Expression：
Tissue_Specificity: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers

Isoforms & Post-Translational Modification：P00533 has four isomers: P00533-1: 134277 Da (predicted); P00533-2: 44664 Da (predicted); P00533-3: 77312 Da (predicted); P00533-4: 69228 Da (predicted).
Phosphorylated on Tyr residues in response to EGF (PubMed:20462955, PubMed:27153536)丨Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting (PubMed:27153536)丨Palmitoylated on Cys residues by ZDHHC20丨Methylated

Subunit：Binding of the ligand triggers homo- and/or heterodimerization of the receptor triggering its autophosphorylation

Documentation

User Guide for Antibodies (1077 KB)