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I-FABP Antibody (YA3770)

(Synonyms: FABPI; I-FABP; MGC133132; FABP2)
Customer Review

Based on 1 Customer Validation

I-FABP Antibody (YA3770) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to I-FABP.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in ascitic fluid containing 0.03% sodium azide.

  • Conjugation:
    Non-conjugated
ALL IHC-P ICC
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • I-FABP Antibody (YA3770) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

I-FABP Antibody (YA3770) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to I-FABP.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 15 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 15 kDa
Immunogen

Purified recombinant fragment of human FABP2 full(aa 1-132).

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in ascitic fluid containing 0.03% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for I-FABP Antibody (YA3770)
    Immunohistochemical analysis of paraffin-embedded human duodenum tissue using I-FABP Antibody (HY-P84073, 1/200). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for I-FABP Antibody (YA3770)
    Immunohistochemical analysis of paraffin-embedded human rectum tissue using I-FABP Antibody (HY-P84073, 1/200). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for I-FABP Antibody (YA3770)
    Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using I-FABP Antibody (HY-P84073, 1/200). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for I-FABP Antibody (YA3770)
    Immunocytochemistry analysis of HepG2 cells labeling FABP2 with FABP2 Antibody (HY-P84073) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP2 Antibody (HY-P84073) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for I-FABP Antibody (YA3770)
    Immunocytochemistry analysis of A431 cells labeling FABP2 with FABP2 Antibody (HY-P84073) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP2 Antibody (HY-P84073)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    I-FABP is a FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:It is expressed in the small intestine, with much lower expression levels in the large intestine. The highest expression levels are found in the jejunum.

    Induction:By EGF

  • SwissProt ID

    P12104

  • Gene ID
    2169 [NCBI]
  • Synonyms

    FABPI; I-FABP; MGC133132; FABP2

I-FABP Antibody (YA3770) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human duodenum tissue using I-FABP Antibody (HY-P84073, 1/200). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human rectum tissue using I-FABP Antibody (HY-P84073, 1/200). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using I-FABP Antibody (HY-P84073, 1/200). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling FABP2 with FABP2 Antibody (HY-P84073) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP2 Antibody (HY-P84073) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling FABP2 with FABP2 Antibody (HY-P84073) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP2 Antibody (HY-P84073)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • I-FABP Antibody (YA3770) Product Image
    1/16
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • I-FABP Antibody (YA3770) Product Image
  • Experimental Validation Results for I-FABP Antibody (YA3770)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human duodenum tissue using I-FABP Antibody (HY-P84073, 1/200). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for I-FABP Antibody (YA3770)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human rectum tissue using I-FABP Antibody (HY-P84073, 1/200). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for I-FABP Antibody (YA3770)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using I-FABP Antibody (HY-P84073, 1/200). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for I-FABP Antibody (YA3770)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling FABP2 with FABP2 Antibody (HY-P84073) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP2 Antibody (HY-P84073) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for I-FABP Antibody (YA3770)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling FABP2 with FABP2 Antibody (HY-P84073) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP2 Antibody (HY-P84073)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • Experimental Validation Results for I-FABP Antibody (YA3770)
  • I-FABP Antibody (YA3770) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human duodenum tissue using I-FABP Antibody (HY-P84073, 1/200). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • I-FABP Antibody (YA3770) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human rectum tissue using I-FABP Antibody (HY-P84073, 1/200). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • I-FABP Antibody (YA3770) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using I-FABP Antibody (HY-P84073, 1/200). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • I-FABP Antibody (YA3770) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling FABP2 with FABP2 Antibody (HY-P84073) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP2 Antibody (HY-P84073) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • I-FABP Antibody (YA3770) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling FABP2 with FABP2 Antibody (HY-P84073) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP2 Antibody (HY-P84073)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • I-FABP Antibody (YA3770) Product Image IHC-P
  • I-FABP Antibody (YA3770) Product Image IHC-P
  • I-FABP Antibody (YA3770) Product Image IHC-P
  • I-FABP Antibody (YA3770) Product Image ICC
  • I-FABP Antibody (YA3770) Product Image ICC