Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Antibody (YA1530)(PBS only)

Cat. No.: HY-P81785A: Fiche technique User Guide for Antibodies
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Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Antibody (YA1530) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PAK1/2/3 (Ser144/Ser141/Ser154).

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Size		Stock
50 μL		Obtenir un devis
100 μL		Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description

Description

Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Antibody (YA1530) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PAK1/2/3 (Ser144/Ser141/Ser154).

Host

Rabbit

Masse moléculaire

Predicted band size: 62 kDa;

Observed band size: 62 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O75914

Gene ID

5063 [NCBI]

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser144 of human PAK1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Pureté	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC) (By similarity)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:Limited to the nervous system. Highly expressed in postmitotic neurons of the cerebral cortex and hippocampus during development and after birth.

Isoforms & Post-Translational Modification：O75914 has 4 isomers: O75914-1: 62310 Da (predicted); O75914-2: 60693 Da (predicted); O75914-3: 64530 Da (predicted); O75914-4: 62913 Da (predicted).
Autophosphorylated when activated by CDC42/p21;Neddylated

Subunit：Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Shows highly specific binding to the SH3 domains of phospholipase C-gamma and of adapter protein NCK.

Research Field

Neuroscience

Synonyms

PAK3; OPHN3; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3

Documentation

User Guide for Antibodies (1077 KB)