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L1CAM Antibody (YA3149)(PBS only)

(Synonyms: L1CAM; CAML1; MIC5; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD antigen CD171)

L1CAM Antibody (YA3149) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to L1CAM.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for L1CAM Antibody (YA3149)(PBS only)
  • L1CAM Antibody (YA3149)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

L1CAM Antibody (YA3149) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to L1CAM.

  • Host Rabbit
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 140 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 140 kDa
Immunogen

Recombinant protein of human L1CAM aa920-1115.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for L1CAM Antibody (YA3149)(PBS only)
    Western blot analysis of extracts from A375 (lane 2(20μg) and A375 (lane 3(40μg) using L1CAM (HY-P83404) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    L1CAM is a Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell projection, growth cone; Cell projection, axon; Cell projection, dendrite

  • Isoforms & Post-Translational Modification

    P32004 has 3 isomers: P32004-1: 140003 Da (predicted); P32004-2: 139517 Da (predicted); P32004-3: 138908 Da (predicted).

  • Subunit

    Interacts with SHTN1; the interaction occurs in axonal growth cones (By similarity). Interacts with isoform 2 of BSG (By similarity)

  • SwissProt ID

    P32004

  • Gene ID
    3897 [NCBI]
  • Synonyms

    L1CAM; CAML1; MIC5; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD antigen CD171

  • Research Field

    Signal Transduction

L1CAM Antibody (YA3149)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A375 (lane 2(20μg) and A375 (lane 3(40μg) using L1CAM (HY-P83404) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • L1CAM Antibody (YA3149)(PBS only) Product Image
    1/16
  • Experimental Validation Results for L1CAM Antibody (YA3149)(PBS only)
  • L1CAM Antibody (YA3149)(PBS only) Product Image
  • Experimental Validation Results for L1CAM Antibody (YA3149)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A375 (lane 2(20μg) and A375 (lane 3(40μg) using L1CAM (HY-P83404) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for L1CAM Antibody (YA3149)(PBS only)
  • L1CAM Antibody (YA3149)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A375 (lane 2(20μg) and A375 (lane 3(40μg) using L1CAM (HY-P83404) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • L1CAM Antibody (YA3149)(PBS only) Product Image WB