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L1CAM Antibody (YA3149)

(Synonyms: L1CAM; CAML1; MIC5; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD antigen CD171)

L1CAM Antibody (YA3149) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to L1CAM.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for L1CAM Antibody (YA3149)
  • L1CAM Antibody (YA3149) Product Image
  • Experimental Validation Results for L1CAM Antibody (YA3149)
  • L1CAM Antibody (YA3149) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

L1CAM Antibody (YA3149) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to L1CAM.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 140 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 140 kDa
Species Reactivity Database

Entrez Gene: 3897 Human

SwissProt: P32004 Human

Immunogen

Recombinant protein of human L1CAM aa920-1115.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105515

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for L1CAM Antibody (YA3149)
    Western blot analysis of extracts from A375 (lane 2(20μg) and A375 (lane 3(40μg) using L1CAM (HY-P83404) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell projection, growth cone; Cell projection, axon; Cell projection, dendrite

  • Isoforms & Post-Translational Modification

    P32004 has 3 isomers: P32004-1: 140003 Da (predicted); P32004-2: 139517 Da (predicted); P32004-3: 138908 Da (predicted).

  • Subunit

    Interacts with SHTN1; the interaction occurs in axonal growth cones (By similarity). Interacts with isoform 2 of BSG (By similarity)

  • SwissProt ID

    P32004

  • Gene ID
    3897 [NCBI]
  • Synonyms

    L1CAM; CAML1; MIC5; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD antigen CD171

  • Research Field

    Signal Transduction

L1CAM Antibody (YA3149) Related Classifications

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    L1CAM Antibody (YA3149) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Western blot analysis of extracts from A375 (lane 2(20μg) and A375 (lane 3(40μg) using L1CAM (HY-P83404) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • L1CAM Antibody (YA3149) Product Image
    1/16
  • Experimental Validation Results for L1CAM Antibody (YA3149)
  • L1CAM Antibody (YA3149) Product Image
  • L1CAM Antibody (YA3149) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A375 (lane 2(20μg) and A375 (lane 3(40μg) using L1CAM (HY-P83404) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • L1CAM Antibody (YA3149) Product Image WB