TPPP Antibody (YA2185)
(Synonyms: p24; p25; p25alpha; TPPP; TPPP/p25; TPPP1; Tubulin polymerization promoting protein)
Based on 1 Customer Validation
TPPP Antibody (YA2185) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TPPP.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:100-1:200 | 1:50-1:200 | 1:50-1:100 |
Product Details
TPPP Antibody (YA2185) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TPPP.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 24 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 24 kDa
Entrez Gene: 11076 Human ; 72948 Mouse ; 361466 Rat
SwissProt: O94811 Human ; Q7TQD2 Mouse ;
OMIM: 608773 Human
A synthesized peptide derived from human TPPP aa100-165.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TPPP is a Regulator of microtubule dynamics that plays a key role in myelination by promoting elongation of the myelin sheath. Acts as a microtubule nucleation factor in oligodendrocytes: specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath. Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes. Shows magnesium-dependent GTPase activity; the role of the GTPase activity is unclear. In addition to microtubule nucleation activity, also involved in microtubule bundling and stabilization of existing microtubules, thereby maintaining the integrity of the microtubule network. Regulates microtubule dynamics by promoting tubulin acetylation: acts by inhibiting the tubulin deacetylase activity of HDAC6. Also regulates cell migration: phosphorylation by ROCK1 inhibits interaction with HDAC6, resulting in decreased acetylation of tubulin and increased cell motility. Plays a role in cell proliferation by regulating the G1/S-phase transition. Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis; this process is regulated by phosphorylation by LIMK2[1][2][3][4][5][6][7][8][9][10][11][12].
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Subcellular Localization
Golgi outpost; Cytoplasm, cytoskeleton, microtubule organizing center; Cytoplasm, cytoskeleton; Nucleus; Cytoplasm, cytoskeleton, spindle
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Expression
Tissue_specificity:Widely expressed
Induction:enriched in cerebrospinal fluid of multiple sclerosis (at protein level) -
Subunit
Homodimer (PubMed:17693641, PubMed:22484033, PubMed:26289831, PubMed:33831707). Binds tubulin; binding is inhibited by GTP (PubMed:17105200, PubMed:26289831). Interacts with MAPK1 (By similarity). Interacts with GAPDH; the interaction is direct (By similarity). Interacts with LIMK1 (via the PDZ domain); the interaction is direct (PubMed:18028908, PubMed:22328514). Interacts with LIMK2 (PubMed:22328514). Interacts with HDAC6; thereby inhibiting the tubulin deacetylase activity of HDAC6 (PubMed:20308065, PubMed:23093407). Interacts with aggregated SNCA; may have a pro-aggregatory role in synucleinopathies (PubMed:15590652, PubMed:17027006). Interacts with DYNLL1 (PubMed:31505170). Interacts (via C-terminus) with S100A2, S100A6 and S100B; these interactions inhibit TPPP dimerization (PubMed:33831707)
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SwissProt ID
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Synonyms
p24; p25; p25alpha; TPPP; TPPP/p25; TPPP1; Tubulin polymerization promoting protein
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Research Field
Signal Transduction
Documentation
[1]. Fu MM, et al. The Golgi Outpost Protein TPPP Nucleates Microtubules and Is Critical for Myelination. Cell. 2019 Sep 19;179(1):132-146.e14. [Content Brief]
[2]. Doi S, et al. Regulation of the tubulin polymerization-promoting protein by Ca(2+)/S100 proteins. Cell Calcium. 2021 Jun;96:102404. [Content Brief]
[3]. Zotter A, et al. Disordered TPPP/p25 binds GTP and displays Mg2+-dependent GTPase activity. FEBS Lett. 2011 Mar 9;585(5):803-8. [Content Brief]
[4]. Zotter Á, et al. Zn²+-induced rearrangement of the disordered TPPP/p25 affects its microtubule assembly and GTPase activity. Biochemistry. 2011 Nov 8;50(44):9568-78. [Content Brief]
[5]. Vincze O, et al. Tubulin polymerization promoting proteins (TPPPs): members of a new family with distinct structures and functions. Biochemistry. 2006 Nov 21;45(46):13818-26. [Content Brief]
[6]. Hlavanda E, et al. Phosphorylation blocks the activity of tubulin polymerization-promoting protein (TPPP): identification of sites targeted by different kinases. J Biol Chem. 2007 Oct 5;282(40):29531-9. [Content Brief]
[7]. Acevedo K, et al. The phosphorylation of p25/TPPP by LIM kinase 1 inhibits its ability to assemble microtubules. Exp Cell Res. 2007 Dec 10;313(20):4091-106. [Content Brief]
[8]. DeBonis S, et al. Self protein-protein interactions are involved in TPPP/p25 mediated microtubule bundling. Sci Rep. 2015 Aug 20;5:13242. [Content Brief]
[9]. Tokési N, et al. TPPP/p25 promotes tubulin acetylation by inhibiting histone deacetylase 6. J Biol Chem. 2010 Jun 4;285(23):17896-906. [Content Brief]
[10]. Schofield AV, et al. Rho-associated coiled-coil kinase (ROCK) protein controls microtubule dynamics in a novel signaling pathway that regulates cell migration. J Biol Chem. 2012 Dec 21;287(52):43620-9. [Content Brief]
[11]. Schofield AV, et al. Tubulin polymerization promoting protein 1 (Tppp1) phosphorylation by Rho-associated coiled-coil kinase (rock) and cyclin-dependent kinase 1 (Cdk1) inhibits microtubule dynamics to increase cell proliferation. J Biol Chem. 2013 Mar 15;288(11):7907-7917. [Content Brief]
[12]. Heng YW, et al. TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation. J Cell Sci. 2012 Mar 15;125(Pt 6):1579-90. [Content Brief]
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