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IL-2 Antibody (YA4115)

(Synonyms: Aldesleukin; TCGF; interleukin-2)
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Based on 1 Customer Validation

IL-2 Antibody (YA4115) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IL-2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for IL-2 Antibody (YA4115)
  • Experimental Validation Results for IL-2 Antibody (YA4115)
  • IL-2 Antibody (YA4115) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:200-1:1000 1:10000

Product Details

Description

IL-2 Antibody (YA4115) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IL-2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 18 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 17.6 kDa
Immunogen

Purified recombinant fragment of IL2 aa 21-153.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718902

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IL-2 Antibody (YA4115)
    Western blot analysis of extracts from C2C12(lane 1(40μg) ),Jukat(lane 2(40μg) )and Hela(lane 3(40μg) ) using IL2 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IL-2 Antibody (YA4115)
    Immunofluorescence analysis of Sk-Br-3 cells labeling IL2 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with IL2 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

Background

  • Function

    IL-2R (Interleukin-2 receptor) is the receptor for Interleukin-2. IL-2R has three component peptides, α, β, γ. And they have different affinities for IL-2: low-affinity IL-2Rα, intermediate-affinity IL-2Rβγ and high-affinity IL-2Rαβγ[1]. IL-2R mediates T cell immune responses, such as stimulating T cell proliferation and activating lymphokine-activated killer cells[2]. IL-2R also mediates IL-2 internalization and signal transduction, such as cell proliferation or differentiation[3].

  • Subcellular Localization

    Secreted

  • SwissProt ID

    P60568

  • Gene ID
    3558 [NCBI]
  • Synonyms

    Aldesleukin; TCGF; interleukin-2

References

[1]. Hodge S, et al. Surface and intracellular interleukin-2 receptor expression on various resting and activated populations involved in cell-mediated immunity in human peripheral blood. Scand J Immunol. 2000 Jan;51(1):67-72. [Content Brief]

[2]. R N Bamfordm, et al. The interleukin (IL) 2 receptor beta chain is shared by IL-2 and a cytokine, provisionally designated IL-T, that stimulates T-cell proliferation and the induction of lymphokine-activated killer cells. Proc Natl Acad Sci U S A.1994 May 24;91(11):4940-4. [Content Brief]

[3]. M Allouche, et al. Interleukin 2 receptors. Leuk Res. 1990;14(8):699-703. [Content Brief]

IL-2 Antibody (YA4115) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C2C12(lane 1(40μg) ),Jukat(lane 2(40μg) )and Hela(lane 3(40μg) ) using IL2 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Sk-Br-3 cells labeling IL2 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with IL2 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • IL-2 Antibody (YA4115) Product Image
    1/16
  • Experimental Validation Results for IL-2 Antibody (YA4115)
  • Experimental Validation Results for IL-2 Antibody (YA4115)
  • IL-2 Antibody (YA4115) Product Image
  • Experimental Validation Results for IL-2 Antibody (YA4115)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C2C12(lane 1(40μg) ),Jukat(lane 2(40μg) )and Hela(lane 3(40μg) ) using IL2 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IL-2 Antibody (YA4115)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Sk-Br-3 cells labeling IL2 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with IL2 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
  • Experimental Validation Results for IL-2 Antibody (YA4115)
  • Experimental Validation Results for IL-2 Antibody (YA4115)
  • IL-2 Antibody (YA4115) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C2C12(lane 1(40μg) ),Jukat(lane 2(40μg) )and Hela(lane 3(40μg) ) using IL2 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • IL-2 Antibody (YA4115) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Sk-Br-3 cells labeling IL2 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with IL2 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • IL-2 Antibody (YA4115) Product Image WB
  • IL-2 Antibody (YA4115) Product Image ICC