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TRPM8 Antibody (YA1609)

(Synonyms: LTrpC6; MGC2849; Transient receptor potential p8; Trp p8; Trpm8; TRPP8)
Customer Review

Based on 1 Customer Validation

TRPM8 Antibody (YA1609) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRPM8.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
  • TRPM8 Antibody (YA1609) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

TRPM8 Antibody (YA1609) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRPM8.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 128 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 128 kDa
Species Reactivity Database

Entrez Gene: 79054 Human ;

SwissProt: Q7Z2W7 Human ;

Immunogen

A synthesized peptide derived from human TRPM8 aa900-1000.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103962

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TRPM8 Antibody (YA1609)
    Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using TRPM8 Antibody (HY-P81864) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
    Immunocytochemistry analysis of A549 cells labeling TRPM8 with TRPM8 Antibody (HY-P81864) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TRPM8 Antibody ((HY-P81864) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
    Immunocytochemistry analysis of A549 cells labeling TRPM8 with TRPM8 Antibody (HY-P81864) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TRPM8 Antibody ((HY-P81864) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    TRPM8 is a cold- and menthol-gated, calcium-permeable ion channel that functions as a primary molecular sensor for cold and menthol in humans and supports cold detection in mammalian sensory neurons[1][2]. Mechanistically, ligand-bound TRPM8 structures identify binding sites for cooling agonists and PI (4,5) P2, indicating that lipid-dependent allosteric control contributes to TRPM8 activation[2]. In mouse models, functional TRPM8 channels are required for innocuous cool responses, noxious cold responses, injury-evoked cold hypersensitivity, cooling-mediated analgesia, and thermoregulation[1]. In disease-relevant models, systemic TRPM8 blockade reduced cold hypersensitivity in inflammatory and nerve-injury pain models, whereas TRPM8 activation by menthol or WS-12 attenuated paclitaxel-induced mechanical pain in rat dorsal root ganglion studies[1][3]. Compared with related isoforms, TRPM8 differs from TRPV1, a heat/capsaicin receptor, and TRPA1, whose role as a direct noxious cold sensor remains controversial; TRPA1 can instead act upstream of GFRα3 and TRPM8 during neurogenic inflammation-driven cold hypersensitivity[4]. For experimental applications, selective antagonists such as PBMC and biphenyl amide analogues, and agonists such as menthol and WS-12, provide tools for testing TRPM8 gating, cold allodynia, sensory neuropathy, and analgesic mechanisms[1][3][5].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Membrane raft; Endoplasmic reticulum membrane

  • Expression


    Tissue_specificity:Expressed in prostate. Also expressed in prostate tumors and in non-prostatic primary tumors such as colon, lung, breast and skin tumors

  • Isoforms & Post-Translational Modification

    Q7Z2W7 has 4 isomers: Q7Z2W7-1: 127685 Da (predicted); Q7Z2W7-2: 5940 Da (predicted); Q7Z2W7-3: 18655 Da (predicted); Q7Z2W7-4: 79096 Da (predicted).

  • Subunit

    Homotetramer (PubMed:37857704). Isoform 2 and isoform 3 interact with the C-terminus of isoform 1 in a thermosensitive manner with decreased interaction at 21 degrees Celsius compared to 37 degrees Celsius (PubMed:22128173).

  • SwissProt ID

    Q7Z2W7

  • Gene ID
    79054 [NCBI]
  • Synonyms

    LTrpC6; MGC2849; Transient receptor potential p8; Trp p8; Trpm8; TRPP8

  • Research Field

    Signal Transduction

References

[1]. Knowlton WM, et al. Pharmacological blockade of TRPM8 ion channels alters cold and cold pain responses in mice. PLoS One. 2011;6(9):e25894. [Content Brief]

[2]. Yin Y, et al. Current View of Ligand and Lipid Recognition by the Menthol Receptor TRPM8. Trends Biochem Sci. 2020 Sep;45(9):806-819. [Content Brief]

[3]. Li WW, et al. Roles of Thermosensitive Transient Receptor Channels TRPV1 and TRPM8 in Paclitaxel-Induced Peripheral Neuropathic Pain. Int J Mol Sci. 2024 May 27;25(11):5813. [Content Brief]

[4]. Yamaki S, et al. Nociceptive afferent phenotyping reveals that transient receptor potential ankyrin 1 promotes cold pain through neurogenic inflammation upstream of the neurotrophic factor receptor GFRα3 and the menthol receptor transient receptor potential melastatin 8. Pain. 2021 Feb 1;162(2):609-618. [Content Brief]

[5]. Journigan VB, et al. Structure-Based Design of Novel Biphenyl Amide Antagonists of Human Transient Receptor Potential Cation Channel Subfamily M Member 8 Channels with Potential Implications in the Treatment of Sensory Neuropathies. ACS Chem Neurosci. 2020 Feb 5;11(3):268-290. [Content Brief]

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TRPM8 Antibody (YA1609) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using TRPM8 Antibody (HY-P81864) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling TRPM8 with TRPM8 Antibody (HY-P81864) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TRPM8 Antibody ((HY-P81864) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling TRPM8 with TRPM8 Antibody (HY-P81864) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TRPM8 Antibody ((HY-P81864) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TRPM8 Antibody (YA1609) Product Image
    1/16
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
  • TRPM8 Antibody (YA1609) Product Image
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using TRPM8 Antibody (HY-P81864) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling TRPM8 with TRPM8 Antibody (HY-P81864) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TRPM8 Antibody ((HY-P81864) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling TRPM8 with TRPM8 Antibody (HY-P81864) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TRPM8 Antibody ((HY-P81864) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
  • Experimental Validation Results for TRPM8 Antibody (YA1609)
  • TRPM8 Antibody (YA1609) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using TRPM8 Antibody (HY-P81864) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • TRPM8 Antibody (YA1609) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling TRPM8 with TRPM8 Antibody (HY-P81864) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TRPM8 Antibody ((HY-P81864) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TRPM8 Antibody (YA1609) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling TRPM8 with TRPM8 Antibody (HY-P81864) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TRPM8 Antibody ((HY-P81864) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TRPM8 Antibody (YA1609) Product Image WB
  • TRPM8 Antibody (YA1609) Product Image ICC
  • TRPM8 Antibody (YA1609) Product Image ICC