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  4. Monoclonal Antibodies Recombinant Antibodies
  5. TRPM8 Antibody (YA1609)

TRPM8 Antibody (YA1609) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRPM8.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Descripciòn

Descripciòn

TRPM8 Antibody (YA1609) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRPM8.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Peso molecular
Predicted band size: 128 kDa;
Observed band size: 128 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human TRPM8 aa900-1000.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
Sensitivity Endogenous Pureza Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Verification Image
ALL WB ICC

  • Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using TRPM8 Antibody (HY-P81864) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of A549 cells labeling TRPM8 with TRPM8 Antibody (HY-P81864) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TRPM8 Antibody ((HY-P81864) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of A549 cells labeling TRPM8 with TRPM8 Antibody (HY-P81864) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TRPM8 Antibody ((HY-P81864) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background
Function：Non-selective ion channel permeable to monovalent and divalent cations, including Na(+), K(+), and Ca(2+), with higher permeability for Ca(2+). Activated by multiple factors, such as temperature, voltage, pressure, and changes in osmolality. Activated by cool temperatures (<23-28 degrees Celsius) and by chemical ligands evoking a sensation of coolness, such as menthol and icilin therefore plays a central role in the detection of environmental cold temperatures (PubMed:15306801, PubMed:15852009, PubMed:16174775, PubMed:25559186, PubMed:37857704). TRPM8 is a voltage-dependent channel; its activation by cold or chemical ligands shifts its voltage thresholds towards physiological membrane potentials, leading to the opening of the channel (PubMed:15306801). In addition to its critical role in temperature sensing, regulates basal tear secretion by sensing evaporation-induced cooling and changes in osmolality (By similarity). May plays a role in prostate cancer cell migration (PubMed:16174775, PubMed:25559186); Negatively regulates menthol- and cold-induced channel activity by stabilizing the closed state of the channel; Negatively regulates menthol- and cold-induced channel activity by stabilizing the closed state of the channel
Subcellular Localization：Cell membrane; Multi-pass membrane protein; Membrane raft; Endoplasmic reticulum membrane
Expression：
Tissue_specificity:Expressed in prostate. Also expressed in prostate tumors and in non-prostatic primary tumors such as colon, lung, breast and skin tumors
Isoforms & Post-Translational Modification：Q7Z2W7 has 4 isomers: Q7Z2W7-1: 127685 Da (predicted); Q7Z2W7-2: 5940 Da (predicted); Q7Z2W7-3: 18655 Da (predicted); Q7Z2W7-4: 79096 Da (predicted).
Subunit：Homotetramer (PubMed:37857704). Isoform 2 and isoform 3 interact with the C-terminus of isoform 1 in a thermosensitive manner with decreased interaction at 21 degrees Celsius compared to 37 degrees Celsius (PubMed:22128173).
RRID
Database

Entrez Gene: 79054 Human ;

SwissProt: Q7Z2W7 Human ;

OMIM: 606678 Human

Research Field

Signal Transduction
Synonyms
LTrpC6; MGC2849; Transient receptor potential p8; Trp p8; Trpm8; TRPP8
Documentación
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

TRPM8 Antibody (YA1609) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:
TRPM8 Antibody (YA1609)
Cat. No.:
HY-P81864
Cantidad:
MCE Japan Authorized Agent:

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