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Acetylated-Lysine Antibody (YA3463)

(Synonyms: Acetylated-Lysine Antibody )
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Based on 1 publication(s) in Google Scholar

Acetylated-Lysine Antibody (YA3463) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetylated-Lysine.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP, ChIP, IF-Tissue

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
  • Acetylated-Lysine Antibody (YA3463) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Acetylated-Lysine Antibody (YA3463)

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
ChIP Info
ChIP: Chromatin Immunoprecipitation
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000 1:20000-1:100000 1:5000-1:20000 Use 5 μg for 25 μg of chromatin 1-2μg/sample

Product Details

Description

Acetylated-Lysine Antibody (YA3463) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetylated-Lysine.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Species independent
Immunogen

Synthetic Acetylated lysine-containing peptide.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3676644

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
    Western blot analysis of extracts from Hela (lane2, 20μg), Hela treated with 0.4μM Trichostatin A for 18 hours (lane3, 20μg) and Hela treated with 1μM Trichostatin A for 18 hours (lane4, 20μg) using Acetylated-Lysine Antibody (HY-P83733). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Acetylated-Lysine Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Acetylated-Lysine Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Acetylated-Lysine is an Acetylated-Lysine refers to a lysine amino acid residue that has been modified by the addition of an acetyl group. This acetylation process is a type of post-translational modification that can significantly alter a protein's structure, function, and interactions. Acetylation of lysine residues is a reversible process, and it plays a crucial role in regulating various cellular processes.

  • Expression


    Positive sample: HeLa cell lysate, HeLa treated with 1μM TSA for 18 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 400nM TSA for 18 hours cell lysate, C6 cell lysate

  • Synonyms

    Acetylated-Lysine Antibody

Acetylated-Lysine Antibody (YA3463) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), Hela treated with 0.4μM Trichostatin A for 18 hours (lane3, 20μg) and Hela treated with 1μM Trichostatin A for 18 hours (lane4, 20μg) using Acetylated-Lysine Antibody (HY-P83733). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Acetylated-Lysine Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Acetylated-Lysine Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Acetylated-Lysine Antibody (YA3463) Product Image
    1/16
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
  • Acetylated-Lysine Antibody (YA3463) Product Image
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), Hela treated with 0.4μM Trichostatin A for 18 hours (lane3, 20μg) and Hela treated with 1μM Trichostatin A for 18 hours (lane4, 20μg) using Acetylated-Lysine Antibody (HY-P83733). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Acetylated-Lysine Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Acetylated-Lysine Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
  • Experimental Validation Results for Acetylated-Lysine Antibody (YA3463)
  • Acetylated-Lysine Antibody (YA3463) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg), Hela treated with 0.4μM Trichostatin A for 18 hours (lane3, 20μg) and Hela treated with 1μM Trichostatin A for 18 hours (lane4, 20μg) using Acetylated-Lysine Antibody (HY-P83733). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Acetylated-Lysine Antibody (YA3463) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Acetylated-Lysine Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Acetylated-Lysine Antibody (YA3463) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Acetylated-Lysine Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Acetylated-Lysine Antibody (YA3463) Product Image WB
  • Acetylated-Lysine Antibody (YA3463) Product Image IHC-P
  • Acetylated-Lysine Antibody (YA3463) Product Image IHC-P