MEK3 Antibody (YA3214)(PBS only)

Art. -Nr.: HY-P83469A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

MEK3 Antibody (YA3214) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MEK3.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Größe		Verfügbarkeit
50 μL		Angebot einholen
100 μL		Angebot einholen

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

MEK3 Antibody (YA3214) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MEK3.

Host

Rabbit

Molekulargewicht

Predicted band size: 39 kDa;

Observed band size: 38-40 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P46734

Gene ID

5606 [NCBI]

Immunogen

Recombinant protein of human MEK3

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Reinheit	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14

Expression：
Tissue_specificity:This gene is highly expressed in skeletal muscle and is also widely expressed in other tissues.

Isoforms & Post-Translational Modification：P46734 has 3 isomers: P46734-1: 39318 Da (predicted); P46734-2: 36173 Da (predicted); P46734-3: 39940 Da (predicted).
Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases positively regulates the kinase activity (PubMed:8622669). Phosphorylated by TAOK2 (PubMed:11279118);(Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway

Subunit：Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK (PubMed:21224381). Binds to DYRK1B/MIRK and increases its kinase activity (PubMed:11980910). Part of a complex with MAP3K3, RAC1 and CCM2 (By similarity). Interacts with ARRB1 (PubMed:16709866)

Research Field

Signal Transduction

Synonyms

AW212142; dual specificity mitogen activated protein kinase kinase 3; Dual specificity mitogen activated protein kinase kinase 6; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAP kinase kinase 6; map2k3; MAP2K6; MAPK ERK kinase 3; MAPK/ERK kinase 3; MAPK/ERK kinase 6; MAPKK 3; MAPKK 6; MAPKK3; MAPKK6; MEK 3; MEK 6; MEK3; Mitogen activated protein kinase kinase 3; Mitogen activated protein kinase kinase 6; MKK 3; MKK3; MKK6; mMKK3b; MP2K3_HUMAN; PRKMK 3; PRKMK3; PRKMK6; Protein kinase; mitogen activated; kinase 6 (MAP kinase kinase 6); protein kinase; mitogen-activated; kinase 3; SAPK kinase 2; SAPKK 2; SAPKK 3; SAPKK-2; SAPKK2; SAPKK3; Stress activated protein kinase kinase 2; Stress activated protein kinase kinase 3; Stress-activated protein kinase kinase 2.

Dokumentation

User Guide for Antibodies (1077 KB)