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GFP Tag Antibody (YA4010)

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Based on 1 Customer Validation

GFP Tag Antibody (YA4010) is a mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to GFP Tag.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    WB, IHC-P, ICC/IF, IP, FC, ELISA

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GFP Tag Antibody (YA4010)
  • GFP Tag Antibody (YA4010) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:200-1:1000 1:100-1:200 1:200-1:400 1:20-1:50 1:10000

Product Details

Description

GFP Tag Antibody (YA4010) is a mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to GFP Tag.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 52 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Immunogen

Purified recombinant fragment of GFP aa 2-243.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

RRID

AB_3718879

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GFP Tag Antibody (YA4010)
    Western blot analysis of GFP tag in recombinant fusion protein (GFP Protein, Aequorea victoriae, E.coli,N-6His, HY-P71461) using GFP Tag Antibody (HY-P84313). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    GFP Tag is an Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin

  • Expression


    Tissue_specificity:Photocell

  • Subunit

    Monomer

  • SwissProt ID

    P42212

GFP Tag Antibody (YA4010) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of GFP tag in recombinant fusion protein (GFP Protein, Aequorea victoriae, E.coli,N-6His, HY-P71461) using GFP Tag Antibody (HY-P84313). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • GFP Tag Antibody (YA4010) Product Image
    1/16
  • Experimental Validation Results for GFP Tag Antibody (YA4010)
  • GFP Tag Antibody (YA4010) Product Image
  • Experimental Validation Results for GFP Tag Antibody (YA4010)
    1/1
    MedChemExpress Validation
     Western blot analysis of GFP tag in recombinant fusion protein (GFP Protein, Aequorea victoriae, E.coli,N-6His, HY-P71461) using GFP Tag Antibody (HY-P84313). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GFP Tag Antibody (YA4010)
  • GFP Tag Antibody (YA4010) Product Image
    MedChemExpress Validation

    Western blot analysis of GFP tag in recombinant fusion protein (GFP Protein, Aequorea victoriae, E.coli,N-6His, HY-P71461) using GFP Tag Antibody (HY-P84313). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • GFP Tag Antibody (YA4010) Product Image WB