NAA10 Antibody (YA4003)
(Synonyms: TE2; ARD1; NATD; ARD1A; ARD1P; OGDNS; DXS707; MCOPS1)NAA10 Antibody (YA4003) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NAA10.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, ICC/IF, FC, ELISA
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Reactivity :
Human, Mouse, Monkey
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
NAA10 Antibody (YA4003) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NAA10.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Monkey
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Observed Molecular WeightObserved band size: 26 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 26 kDa
Purified recombinant fragment of human NAA10 (AA: 111-235) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NAA10 is a Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity. Acetylates amino termini that are devoid of initiator methionine. The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development. Acetylates HSPA1A and HSPA1B at 'Lys-77' which enhances its chaperone activity and leads to preferential binding to co-chaperone HOPX. Acetylates HIST1H4A. Acts as a negative regulator of sister chromatid cohesion during mitosis[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Cytoplasm; Nucleus
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Expression
Tissue_specificity:Ubiquitous -
Isoforms & Post-Translational Modification
P41227 has 2 isomers: P41227-1: 26459 Da (predicted); P41227-2: 24784 Da (predicted).
Cleaved by caspases during apoptosis;Phosphorylation by IKBKB/IKKB at Ser-209 promotes its proteasome-mediated degradation;Autoacetylated at Lys-136 which stimulates its catalytic activity -
Subunit
Component of the N-terminal acetyltransferase A complex (also called the NatA complex) composed of NAA10 and NAA15 (PubMed:15496142, PubMed:20154145, PubMed:32042062). Within the complex interacts with NAA15 (PubMed:15496142, PubMed:20154145, PubMed:29754825, PubMed:32042062). Component of the N-terminal acetyltransferase A (NatA)/HYPK complex at least composed of NAA10, NAA15 and HYPK, which has N-terminal acetyltransferase activity (PubMed:20154145, PubMed:29754825, PubMed:32042062). In complex with NAA15, interacts with HYPK (PubMed:20154145, PubMed:29754825, PubMed:32042062). Component of the N-terminal acetyltransferase E (NatE) complex at least composed of NAA10, NAA15 and NAA50 (PubMed:29754825, PubMed:32042062). Within the complex interacts with NAA15; the interaction is required for binding to NAAT50 (PubMed:29754825, PubMed:32042062). Interacts with NAAT50 (PubMed:16507339, PubMed:32042062). The interaction of the NatA complex with NAA50 reduces the acetylation activity of the NatA complex (PubMed:32042062). Component of the N-terminal acetyltransferase E (NatE)/HYPK complex at least composed of NAA10, NAA15, NAA50 and HYPK (PubMed:32042062). In complex with NAA15, interacts with HYPK; the interaction with HYPK reduces the capacity of the NatA complex to interact with NAA50 (PubMed:29754825, PubMed:32042062). Interacts with HIF1A (via its ODD domain); the interaction increases HIF1A protein stability during normoxia, an down-regulates it when induced by hypoxia (PubMed:12464182). Interacts with the ribosome (PubMed:16507339). Binds to MYLK (PubMed:19826488). Interacts with NAA16 (PubMed:19480662). Interacts (via its C-terminal domain) with TSC2, leading to its acetylation (PubMed:20145209). Interacts with IKBKB (PubMed:19716809). Interacts with HSPA1A and HSPA1B leading to its acetylation (PubMed:27708256)
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SwissProt ID
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Synonyms
TE2; ARD1; NATD; ARD1A; ARD1P; OGDNS; DXS707; MCOPS1
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