BAFF Antibody (YA4582)(PBS only)

Cat. No.: HY-P84885A: Data Sheet User Guide for Antibodies
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BAFF Antibody (YA4582) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to BAFF.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

BAFF Antibody (YA4582) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to BAFF.

Host

Mouse

Molecular Weight

Predicted band size: 31 kDa;

Observed band size: 31 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9Y275

Gene ID

10673 [NCBI]

Immunogen

Purified recombinant fragment of human TNFSF13B (AA: 116-278) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response; Isoform 2 seems to inhibit isoform 1 secretion and bioactivity; Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis

Subcellular Localization：Cell membrane; Single-pass type II membrane protein; Secreted

Expression：
Tissue_specificity:This gene is highly expressed in peripheral blood leukocytes, particularly in monocytes and macrophages. It is also expressed in the spleen, lymph nodes, bone marrow, T cells, and dendritic cells. Expression is lower in the placenta, heart, lungs, fetal liver, thymus, and pancreas. Isomer 2 is expressed in various myeloid cell lines.

Induction:Up-regulated by exposure to IFNG/IFN-gamma. Down-regulated by phorbol myristate acetate/ionomycin treatment

Isoforms & Post-Translational Modification：Q9Y275 has 3 isomers: Q9Y275-1: 31223 Da (predicted); Q9Y275-2: 29137 Da (predicted); Q9Y275-3: 17578 Da (predicted).
The soluble form derives from the membrane form by proteolytic processing;Isoform 2 is not efficiently shed from the membrane unlike isoform 1;N-glycosylated

Subunit：Homotrimer. Isoform 2 heteromultimerizes with isoform 1, probably limiting the amount of functional isoform 1 on the cell surface. Isoform 3 is unlikely form trimers or bind to BAFF receptors

Synonyms

DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNLG7A; TNFSF20

Documentation

User Guide for Antibodies (1077 KB)