A-RAF Antibody (YA2673)(PBS only)

Cat. No.: HY-P82928A: Data Sheet User Guide for Antibodies
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A-RAF Antibody (YA2673) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to A-RAF.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

A-RAF Antibody (YA2673) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to A-RAF.

Host

Rabbit

Molecular Weight

Predicted band size: 68 kDa;

Observed band size: 68 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P10398

Gene ID

369 [NCBI]

Immunogen

Recombinant protein of human A RAF

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2 (PubMed:36402789); Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation

Expression：
Tissue_specificity:Mainly distributed in urogenital tissues

Isoforms & Post-Translational Modification：P10398 has 2 isomers: P10398-1: 67585 Da (predicted); P10398-2: 20904 Da (predicted).
Dephosphorylation of Ser-214 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC); this relieves inactivation and stimulates kinase activity

Subunit：Interacts with TH1L/NELFD

Research Field

Signal Transduction

Synonyms

ARAF; ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks

Documentation

User Guide for Antibodies (1077 KB)