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CD169 Antibody (YA1293)

(Synonyms: CD169 antibody; CD169 antigen antibody; dJ1009E24.1 antibody; DKFZp667F058 antibody; FLJ00051 antibody; FLJ00055 antibody; FLJ00073 antibody; FLJ00411 antibody; FLJ32150 antibody; Sialic acid binding Ig like lectin 1 antibody; sialic acid binding Ig-like lectin 1, sialoadhesin antibody; Sialic acid binding immunoglobulin like lectin 1 antibody; Sialic acid-binding Ig-like lectin 1 antibody; Sialoadhesin antibody; Siglec 1 antibody; SN antibody; SN_HUMAN antibody)
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Based on 1 Customer Validation

CD169 Antibody (YA1293) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD169.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD169 Antibody (YA1293)
  • CD169 Antibody (YA1293) Product Image
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:2000 1:2000

Product Details

Description

CD169 Antibody (YA1293) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD169.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 220 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 183 kDa；
Species Reactivity Database

Entrez Gene: 6614 Human

SwissProt: Q9BZZ2 Human

Immunogen

Recombinant protein within human CD169 aa 1-1,000 / 1,709.

Sensitivity

Endogenous

Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103660

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD169 Antibody (YA1293)
    Western blot analysis of extracts from K562(lane 2(20ug), K562(lane 3(40ug) and RAW264.7(lane 4(20ug),RAW264.7(lane 5(40ug) using CD169Antibody(HY-P81535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    CD169 is a Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Plays a crucial role in limiting bacterial dissemination by engaging sialylated bacteria to promote effective phagocytosis and antigen presentation for the adaptive immune response. Mediates the uptake of various enveloped viruses via sialic acid recognition and subsequently induces the formation of intracellular compartments filled with virions (VCCs). In turn, enhances macrophage-to-T-cell transmission of several viruses including HIV-1 or SARS-CoV-2. Acts as an endocytic receptor mediating clathrin dependent endocytosis. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis. Plays a role in the inhibition of antiviral innate immune by promoting TBK1 degradation via TYROBP and TRIM27-mediated ubiquitination; (Microbial infection) Facilitates viral cytoplasmic entry into activated dendritic cells via recognition of sialylated gangliosides pesent on viral membrane[1][2][3][4][5].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Secreted

  • Expression


    Tissue_specificity:This gene is expressed in macrophages in various tissues. It is found in higher concentrations in macrophages of the spleen, lymph nodes, and perivascular macrophages of the brain, and in lower concentrations in bone marrow, Kupffer cells of the liver, colon, and lamina propria of the lungs. Inflammatory macrophages in rheumatoid arthritis also express this gene.

    Induction:By interferon-alpha (PubMed:28129379) . By viral infection (PubMed:26358190)

    Positive sample: K-562 cell lysate, RAW264.7 cell lysate, THP-1 cells treated with 50ng/mL PMA for 72 hours add 1μg/mL LPS for 24 hours, RAW264.7 cells treated with 1μg/mL LPS for 24 hours.

  • Isoforms & Post-Translational Modification

    Q9BZZ2 has 3 isomers: Q9BZZ2-1: 182624 Da (predicted); Q9BZZ2-2: 175727 Da (predicted); Q9BZZ2-3: 181733 Da (predicted).

  • Subunit

    Interacts with TYROBP. Interacts with CLEC10A (By similarity)

  • SwissProt ID

    Q9BZZ2

  • Gene ID
    6614 [NCBI]
  • Synonyms

    CD169 antibody; CD169 antigen antibody; dJ1009E24.1 antibody; DKFZp667F058 antibody; FLJ00051 antibody; FLJ00055 antibody; FLJ00073 antibody; FLJ00411 antibody; FLJ32150 antibody; Sialic acid binding Ig like lectin 1 antibody; sialic acid binding Ig-like lectin 1, sialoadhesin antibody; Sialic acid binding immunoglobulin like lectin 1 antibody; Sialic acid-binding Ig-like lectin 1 antibody; Sialoadhesin antibody; Siglec 1 antibody; SN antibody; SN_HUMAN antibody

  • Research Field

    Cell biology

CD169 Antibody (YA1293) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug), K562(lane 3(40ug) and RAW264.7(lane 4(20ug),RAW264.7(lane 5(40ug) using CD169Antibody(HY-P81535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD169 Antibody (YA1293) Product Image
    1/16
  • Experimental Validation Results for CD169 Antibody (YA1293)
  • CD169 Antibody (YA1293) Product Image
  • Experimental Validation Results for CD169 Antibody (YA1293)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug), K562(lane 3(40ug) and RAW264.7(lane 4(20ug),RAW264.7(lane 5(40ug) using CD169Antibody(HY-P81535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD169 Antibody (YA1293)
  • CD169 Antibody (YA1293) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20ug), K562(lane 3(40ug) and RAW264.7(lane 4(20ug),RAW264.7(lane 5(40ug) using CD169Antibody(HY-P81535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD169 Antibody (YA1293) Product Image WB