CD169 Antibody (YA1293)

Cat. No.: HY-P81548: Data Sheet COA
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User Guide for Antibodies
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CD169 Antibody (YA1293) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD169.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CD169 Antibody (YA1293) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

CD169 Antibody (YA1293) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD169.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 183 kDa；

Observed band size: 220 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q9BZZ2

Gene ID

6614 [NCBI]

Immunogen

Recombinant protein within human CD169 aa 1-1,000 / 1,709.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
FC FC: Flow Cytometry	1:2000

Sensitivity	Endogenous	Purity	Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from K562(lane 2(20ug), K562(lane 3(40ug) and RAW264.7(lane 4(20ug),RAW264.7(lane 5(40ug) using CD169Antibody(HY-P81535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Plays a crucial role in limiting bacterial dissemination by engaging sialylated bacteria to promote effective phagocytosis and antigen presentation for the adaptive immune response (PubMed:12940982, PubMed:33489013). Mediates the uptake of various enveloped viruses via sialic acid recognition and subsequently induces the formation of intracellular compartments filled with virions (VCCs) (PubMed:28129379). In turn, enhances macrophage-to-T-cell transmission of several viruses including HIV-1 or SARS-CoV-2 (PubMed:28129379, PubMed:34782760). Acts as an endocytic receptor mediating clathrin dependent endocytosis. Preferentially binds to alpha-2,3-linked sialic acid (PubMed:12940982). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. Plays a role in the inhibition of antiviral innate immune by promoting TBK1 degradation via TYROBP and TRIM27-mediated ubiquitination (PubMed:26358190); (Microbial infection) Facilitates viral cytoplasmic entry into activated dendritic cells via recognition of sialylated gangliosides pesent on viral membrane

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Secreted

Expression：
Tissue_specificity:This gene is expressed in macrophages in various tissues. It is found in higher concentrations in macrophages of the spleen, lymph nodes, and perivascular macrophages of the brain, and in lower concentrations in bone marrow, Kupffer cells of the liver, colon, and lamina propria of the lungs. Inflammatory macrophages in rheumatoid arthritis also express this gene.

Induction:By interferon-alpha (PubMed:28129379) . By viral infection (PubMed:26358190)

Positive sample: K-562 cell lysate, RAW264.7 cell lysate, THP-1 cells treated with 50ng/mL PMA for 72 hours add 1μg/mL LPS for 24 hours, RAW264.7 cells treated with 1μg/mL LPS for 24 hours.

Isoforms & Post-Translational Modification：Q9BZZ2 has 3 isomers: Q9BZZ2-1: 182624 Da (predicted); Q9BZZ2-2: 175727 Da (predicted); Q9BZZ2-3: 181733 Da (predicted).

Subunit：Interacts with TYROBP. Interacts with CLEC10A (By similarity)

RRID

AB_3103660

Database

Entrez Gene: 6614 Human

SwissProt: Q9BZZ2 Human

OMIM: 600751 Human

Research Field

Cell biology

Synonyms

CD169 antibody; CD169 antigen antibody; dJ1009E24.1 antibody; DKFZp667F058 antibody; FLJ00051 antibody; FLJ00055 antibody; FLJ00073 antibody; FLJ00411 antibody; FLJ32150 antibody; Sialic acid binding Ig like lectin 1 antibody; sialic acid binding Ig-like lectin 1, sialoadhesin antibody; Sialic acid binding immunoglobulin like lectin 1 antibody; Sialic acid-binding Ig-like lectin 1 antibody; Sialoadhesin antibody; Siglec 1 antibody; SN antibody; SN_HUMAN antibody

Documentation

Select Batch:

Data Sheet (235 KB) SDS (313 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

CD169 Antibody (YA1293) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD169 Antibody (YA1293)

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Background

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CD169 Antibody (YA1293) Related Classifications

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