Periostin Antibody (YA1280)

Cat. No.: HY-P81535: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Periostin Antibody (YA1280) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Periostin.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Periostin Antibody (YA1280) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Periostin Antibody (YA1280) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Periostin.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 93 kDa;

Observed band size: 93 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q15063

Gene ID

10631 [NCBI]

Immunogen

A synthesized peptide derived from human Periostin

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) , SH-SY5Y(lane 3(40ug) and HEK293T(lane 4(20ug) , HEK293T(lane 5(40ug) using Periostin Antibody (HY-P81535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity)

Subcellular Localization：Golgi apparatus; Secreted; Secreted, extracellular space, extracellular matrix

Expression：
Tissue_specificity:It is widely expressed, with the highest expression levels in the aorta, stomach, lower digestive tract, placenta, uterus, thyroid tissue, and breast. It is also expressed in the kidneys (PubMed: 21763681) and lungs (PubMed: 22079858) . Its expression is upregulated in ovarian epithelial tumors. It is not expressed in normal ovaries. It is also highly expressed around the tumor in lung cancer tissue, but not within the tumor itself. It is overexpressed in breast cancer.

Isoforms & Post-Translational Modification：Q15063 has 10 isomers: Q15063-1: 93314 Da (predicted); Q15063-2: 87021 Da (predicted); Q15063-3: 87254 Da (predicted); Q15063-4: 83850 Da (predicted); Q15063-5: 90424 Da (predicted); Q15063-6: 83527 Da (predicted); Q15063-7: 80357 Da (predicted); Q15063-8: 90143 Da (predicted); Q15063-9: 90010 Da (predicted); Q15063-10: 86840 Da (predicted).
Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833)

Subunit：Homodimer (PubMed:29754429). Interacts with BMP1 and fibronectin

RRID

AB_3103649

Database

Entrez Gene: 10631 Human

SwissProt: Q15063 Human

OMIM: 608777 Human

Research Field

Signal Transduction

Synonyms

Fasciclin-I like; OSF2; Periostin; Periostin osteoblast specific factor; PN; POSTN; RP11 412K4.1

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Periostin Antibody (YA1280) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Periostin Antibody (YA1280)

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Background

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Periostin Antibody (YA1280) Related Classifications

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