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Periostin Antibody (YA1280)

(Synonyms: Fasciclin-I like; OSF2; Periostin; Periostin osteoblast specific factor; PN; POSTN; RP11 412K4.1)
Customer Review

Based on 1 Customer Validation

Periostin Antibody (YA1280) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Periostin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Periostin Antibody (YA1280) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:100-1:200

Product Details

Description

Periostin Antibody (YA1280) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Periostin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 93 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 93 kDa
Species Reactivity Database

Entrez Gene: 10631 Human

SwissProt: Q15063 Human

Immunogen

A synthesized peptide derived from human Periostin

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103649

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Periostin Antibody (YA1280)
    Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) , SH-SY5Y(lane 3(40ug) and HEK293T(lane 4(20ug) , HEK293T(lane 5(40ug) using Periostin Antibody (HY-P81535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Periostin Antibody (YA1280)
    Immunohistochemical analysis of paraffin-embedded human oral cancer tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Periostin Antibody (YA1280)
    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Periostin Antibody (YA1280)
    Immunohistochemical analysis of paraffin-embedded human breast tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Periostin Antibody (YA1280)
    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Periostin Antibody (YA1280)
    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Periostin Antibody (YA1280)
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Periostin induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX[1].

  • Subcellular Localization

    Golgi apparatus; Secreted; Secreted, extracellular space, extracellular matrix

  • Expression


    Tissue_specificity:It is widely expressed, with the highest expression levels in the aorta, stomach, lower digestive tract, placenta, uterus, thyroid tissue, and breast. It is also expressed in the kidneys (PubMed: 21763681) and lungs (PubMed: 22079858) . Its expression is upregulated in ovarian epithelial tumors. It is not expressed in normal ovaries. It is also highly expressed around the tumor in lung cancer tissue, but not within the tumor itself. It is overexpressed in breast cancer.

  • Isoforms & Post-Translational Modification

    Q15063 has 10 isomers: Q15063-1: 93314 Da (predicted); Q15063-2: 87021 Da (predicted); Q15063-3: 87254 Da (predicted); Q15063-4: 83850 Da (predicted); Q15063-5: 90424 Da (predicted); Q15063-6: 83527 Da (predicted); Q15063-7: 80357 Da (predicted); Q15063-8: 90143 Da (predicted); Q15063-9: 90010 Da (predicted); Q15063-10: 86840 Da (predicted).
    Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833)

  • Subunit

    Homodimer (PubMed:29754429). Interacts with BMP1 and fibronectin

  • SwissProt ID

    Q15063

  • Gene ID
    10631 [NCBI]
  • Synonyms

    Fasciclin-I like; OSF2; Periostin; Periostin osteoblast specific factor; PN; POSTN; RP11 412K4.1

  • Research Field

    Signal Transduction

References

[1]. Gillan L, et al. Periostin secreted by epithelial ovarian carcinoma is a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins and promotes cell motility. Cancer Res. 2002 Sep 15;62(18):5358-64. [Content Brief]

Periostin Antibody (YA1280) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) , SH-SY5Y(lane 3(40ug) and HEK293T(lane 4(20ug) , HEK293T(lane 5(40ug) using Periostin Antibody (HY-P81535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human oral cancer tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Periostin Antibody (YA1280) Product Image
    1/16
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Periostin Antibody (YA1280) Product Image
  • Experimental Validation Results for Periostin Antibody (YA1280)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) , SH-SY5Y(lane 3(40ug) and HEK293T(lane 4(20ug) , HEK293T(lane 5(40ug) using Periostin Antibody (HY-P81535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Periostin Antibody (YA1280)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human oral cancer tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Periostin Antibody (YA1280)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Periostin Antibody (YA1280)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Periostin Antibody (YA1280)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Periostin Antibody (YA1280)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Periostin Antibody (YA1280)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Experimental Validation Results for Periostin Antibody (YA1280)
  • Periostin Antibody (YA1280) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) , SH-SY5Y(lane 3(40ug) and HEK293T(lane 4(20ug) , HEK293T(lane 5(40ug) using Periostin Antibody (HY-P81535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Periostin Antibody (YA1280) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human oral cancer tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Periostin Antibody (YA1280) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Periostin Antibody (YA1280) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Periostin Antibody (YA1280) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Periostin Antibody (YA1280) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Periostin Antibody (YA1280) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Periostin Antibody (HY-P81535, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Periostin Antibody (YA1280) Product Image WB
  • Periostin Antibody (YA1280) Product Image IHC-P
  • Periostin Antibody (YA1280) Product Image IHC-P
  • Periostin Antibody (YA1280) Product Image IHC-P
  • Periostin Antibody (YA1280) Product Image IHC-P
  • Periostin Antibody (YA1280) Product Image IHC-P
  • Periostin Antibody (YA1280) Product Image IHC-P