MIB1 Antibody (YA4360)(PBS only)
(Synonyms: MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2)MIB1 Antibody (YA4360) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MIB1.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, ICC/IF, FC, ELISA
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Reactivity :
Human, Monkey
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
MIB1 Antibody (YA4360) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MIB1.
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Host Mouse
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Species ReactivityHuman, Monkey
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Observed Molecular WeightObserved band size: 110 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 110 kDa
Purified recombinant fragment of human MIB1 (AA: 6-221) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MIB1 is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation; (Microbial infection) During adenovirus infection, mediates ubiquitination of Core-capsid bridging protein. This allows viral genome delivery into nucleus for infection
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Subcellular Localization
Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite; Cell membrane
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Expression
Tissue_specificity:It is widely expressed, but at low levels. Higher expression levels were observed in the spinal cord, ovaries, whole brain, and all specific brain regions examined. -
Subunit
Interacts with CEP131 and PCM1
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SwissProt ID
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Synonyms
MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2
Documentation
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