APG5L Antibody

Cat. No.: HY-P80545: FAQs
Data Sheet SDS COA User Guide for Antibodies

APG5L Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 32 kDa, targeting to APG5L. It can be used for WB,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	APG5L Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 32 kDa, targeting to APG5L. It can be used for WB,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat.
Background	APG5L: The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Tag	Free
Gene ID	9474
Synonyms	APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; Apoptosis specific protein; ASP; ATG 5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5
Molecular Weight	Predicted band size: 32 kDa；Observed band size: 55 kDa
Purity	affinity purified
Conjugation	Non-conjugated
Research Field	Cell Biology
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Host	Rabbit
Reactivity	Human, Mouse, Rat
SwissProt ID	Q9H1Y0
Dilution Ratio	WB: 1:500-1:1,000 ;IF: 1:50-1:200 ;IP: 1:20 Western blot analysis of extracts from Raji(lane 2(20μg) ), Hela(lane 3(20μg) ) ,RAW264.7(lane 4(20μg) ) and NIH3T3(lane 5(20μg)) using APG5L(HY-P80545) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80545, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Application	WB, ICC/IF, IP
Appearance	Liquid
Formulation	Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Immunogen	Synthetic peptide corresponding to Human APG5L/ATG5.The exact sequence is proprietary to MCE.
Documentation	Select Batch: Data Sheet (234 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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APG5L Antibody

Biological Activity

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Properties

Documentation

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