PCBP2 Antibody (YA1543)

Cat. No.: HY-P81798: Data Sheet COA
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User Guide for Antibodies
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PCBP2 Antibody (YA1543) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PCBP2.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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PCBP2 Antibody (YA1543) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
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PCBP2 Antibody (YA1543) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PCBP2.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 39 kDa;

Observed band size: 35-45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse, Rat

SwissProt ID

Q15366

Gene ID

5094 [NCBI]

Immunogen

A synthetic peptide of human PCBP2 aa275-288.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from C6 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using PCBP2 (HY-P81798) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Single-stranded nucleic acid binding protein that binds preferentially to oligo dC (PubMed:12414943, PubMed:7607214). Major cellular poly(rC)-binding protein (PubMed:12414943). Also binds poly(rU) (PubMed:12414943). Acts as a negative regulator of antiviral signaling (PubMed:19881509, PubMed:35322803). Negatively regulates cellular antiviral responses mediated by MAVS signaling (PubMed:19881509). It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation (PubMed:19881509). Negativeley regulates the cGAS-STING pathway via interaction with CGAS, preventing the formation of liquid-like droplets in which CGAS is activated (PubMed:35322803). Together with PCBP1, required for erythropoiesis, possibly by regulating mRNA splicing (By similarity); (Microbial infection) In case of infection by poliovirus, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:12414943, PubMed:24371074). Also plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed:12414943)

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:Detected in all tissues examined

Isoforms & Post-Translational Modification：Q15366 has 8 isomers: Q15366-1: 38580 Da (predicted); Q15366-2: 38651 Da (predicted); Q15366-3: 38222 Da (predicted); Q15366-4: 34917 Da (predicted); Q15366-5: 35347 Da (predicted); Q15366-6: 38151 Da (predicted); Q15366-7: 33497 Da (predicted); Q15366-8: 33926 Da (predicted).
Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity;(Microbial infection) Proteolytically cleaved by picornavirus proteinase 3CD

Subunit：Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1 (PubMed:19029303). Interacts with IFIH1 and RNF135 (PubMed:19881509). Interacts with MAVS (via C-terminus) and ITCH (via WW domains) (PubMed:19881509). Interacts with CGAS; preventing the formation of liquid-like droplets in which CGAS is activated (PubMed:35322803)

RRID

AB_3103899

Database

Entrez Gene: 5094 Human ; 18521 Mouse ; 363005 Rat

SwissProt: Q15366 Human ; Q61990 Mouse ;

OMIM: 601210 Human

Research Field

Microbiology

Synonyms

HNRPE2; HNRNPE2; hnRNP-E2

각종 서류

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

PCBP2 Antibody (YA1543) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PCBP2 Antibody (YA1543)

PCBP2 Antibody (YA1543) Featured Recommendations:

Product Detail

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Background

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PCBP2 Antibody (YA1543) Related Classifications

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