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PCBP2 Antibody (YA1543)

(Synonyms: HNRPE2; HNRNPE2; hnRNP-E2)

PCBP2 Antibody (YA1543) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PCBP2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PCBP2 Antibody (YA1543)
  • PCBP2 Antibody (YA1543) Product Image
  • Experimental Validation Results for PCBP2 Antibody (YA1543)
  • PCBP2 Antibody (YA1543) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Descripciòn

PCBP2 Antibody (YA1543) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PCBP2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 35-45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human PCBP2 aa275-288.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103899

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Envío

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PCBP2 Antibody (YA1543)
    Western blot analysis of extracts from C6 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using PCBP2 (HY-P81798) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Single-stranded nucleic acid binding protein that binds preferentially to oligo dC (PubMed:12414943, PubMed:7607214). Major cellular poly(rC)-binding protein (PubMed:12414943). Also binds poly(rU) (PubMed:12414943). Acts as a negative regulator of antiviral signaling (PubMed:19881509, PubMed:35322803). Negatively regulates cellular antiviral responses mediated by MAVS signaling (PubMed:19881509). It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation (PubMed:19881509). Negativeley regulates the cGAS-STING pathway via interaction with CGAS, preventing the formation of liquid-like droplets in which CGAS is activated (PubMed:35322803). Together with PCBP1, required for erythropoiesis, possibly by regulating mRNA splicing (By similarity); (Microbial infection) In case of infection by poliovirus, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:12414943, PubMed:24371074). Also plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed:12414943)

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:Detected in all tissues examined

  • Isoforms & Post-Translational Modification

    Q15366 has 8 isomers: Q15366-1: 38580 Da (predicted); Q15366-2: 38651 Da (predicted); Q15366-3: 38222 Da (predicted); Q15366-4: 34917 Da (predicted); Q15366-5: 35347 Da (predicted); Q15366-6: 38151 Da (predicted); Q15366-7: 33497 Da (predicted); Q15366-8: 33926 Da (predicted).
    Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity;(Microbial infection) Proteolytically cleaved by picornavirus proteinase 3CD

  • Subunit

    Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1 (PubMed:19029303). Interacts with IFIH1 and RNF135 (PubMed:19881509). Interacts with MAVS (via C-terminus) and ITCH (via WW domains) (PubMed:19881509). Interacts with CGAS; preventing the formation of liquid-like droplets in which CGAS is activated (PubMed:35322803)

  • SwissProt ID

    Q15366

  • Gene ID
    5094 [NCBI]
  • Synonyms

    HNRPE2; HNRNPE2; hnRNP-E2

  • Research Field

    Microbiology

PCBP2 Antibody (YA1543) Related Classifications

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    PCBP2 Antibody (YA1543) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using PCBP2 (HY-P81798) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PCBP2 Antibody (YA1543) Product Image
    1/16
  • Experimental Validation Results for PCBP2 Antibody (YA1543)
  • PCBP2 Antibody (YA1543) Product Image
  • PCBP2 Antibody (YA1543) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using PCBP2 (HY-P81798) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PCBP2 Antibody (YA1543) Product Image WB