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  5. PCBP1 Antibody (YA2927)

PCBP1 Antibody (YA2927) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PCBP1.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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제품 설명

PCBP1 Antibody (YA2927) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PCBP1.
Host

Rabbit
Clonality

Recombinant, Monoclonal
분자량
Predicted band size: 37 kDa;
Observed band size: 40 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human PCBP1 aa11-65.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IP
IP: Immunoprecipitation
1:50
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), NIH/3T3 (lane 3(40μg), using PCBP1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background
Function：Single-stranded nucleic acid binding protein that binds preferentially to oligo dC (PubMed:15731341, PubMed:7556077, PubMed:7607214, PubMed:8152927). Together with PCBP2, required for erythropoiesis, possibly by regulating mRNA splicing (By similarity); (Microbial infection) In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD
Subcellular Localization：Nucleus; Cytoplasm
Expression：
Tissue_specificity:Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands
RRID
Database

Entrez Gene: 5093 Human ; 23983 Mouse ; 500242 Rat

SwissProt: Q15365 Human ; P60335 Mouse ;

OMIM: 601209 Human

Research Field

-
Synonyms
Alpha-CP1; hnRNP E1; HNRPE1; HNRPX; PCBP1
각종 서류
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

PCBP1 Antibody (YA2927) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
PCBP1 Antibody (YA2927)
Cat. No.:
HY-P83182
수량:
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SAFETY DATA SHEET (SDS)

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