GATA4 Antibody (YA4873)
(Synonyms: GATA4; Transcription factor GATA-4; GATA-binding factor 4)GATA4 Antibody (YA4873) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to GATA4.
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Host:
Mouse
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Application:
WB, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:10000 |
Product Details
GATA4 Antibody (YA4873) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to GATA4.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse
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Calculated Molecular Weight Predicted band size: 45 kDa;
Purified recombinant fragment of human GATA-4 expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
GATA4 transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function. In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase[1][2][3][4][5][6].
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Subcellular Localization
Nucleus
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Isoforms & Post-Translational Modification
P43694 has 2 isomers: P43694-1: 44565 Da (predicted); P43694-2: 44665 Da (predicted).
Methylation at Lys-300 attenuates transcriptional activity -
Subunit
Interacts with ZNF260 (By similarity). Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts (via the second Zn finger) with NFATC4 (By similarity). Interacts with LMCD1 (By similarity). Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes. Interacts with NR5A1, ZFPM2 and TBX5. Interacts with TBX18. Interacts with GLYR1; the interaction is required for a synergistic activation of GATA4 target genes transcription (PubMed:35182466). Interacts with PHF7; the interaction promotes GATA4 binding to its transcription targets (By similarity)
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SwissProt ID
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Synonyms
GATA4; Transcription factor GATA-4; GATA-binding factor 4
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