Phospholipase C beta 3 Antibody (YA2694)

Cat. No.: HY-P82949: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Phospholipase C beta 3 Antibody (YA2694) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospholipase C beta 3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospholipase C beta 3 Antibody (YA2694) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Phospholipase C beta 3 Antibody (YA2694) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospholipase C beta 3.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 139 kDa;

Observed band size: 150 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q01970

Gene ID

5331 [NCBI]

Immunogen

A synthesized peptide derived from human PLCB3 aa1165-1200.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from A431(lane 2(20μg)) ,Hela (lane 3(20μg)) and HepG2(lane 4(20μg)) using Phospholipase C beta 3 (HY-P82949) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Catalyzes the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed:20966218, PubMed:29122926, PubMed:37991948, PubMed:9188725). Key transducer of G protein-coupled receptor signaling: activated by G(q)/G(11) G alpha proteins downstream of G protein-coupled receptors activation (PubMed:20966218, PubMed:37991948). In neutrophils, participates in a phospholipase C-activating N-formyl peptide-activated GPCR (G protein-coupled receptor) signaling pathway by promoting RASGRP4 activation by DAG, to promote neutrophil functional responses (By similarity)

Subcellular Localization：Cytoplasm; Membrane; Nucleus

Isoforms & Post-Translational Modification：Q01970 has 2 isomers: Q01970-1: 138799 Da (predicted); Q01970-2: 131205 Da (predicted).

Subunit：Interacts with SHANK2 (By similarity). Interacts with LPAR2 (PubMed:15143197)

RRID

AB_3105068

Database

Entrez Gene: 5331 Human

SwissProt: Q01970 Human

OMIM: 618961 Human

Research Field

Signal Transduction

Synonyms

PLCB3; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3

Documentation

Select Batch:

Data Sheet (232 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospholipase C beta 3 Antibody (YA2694) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospholipase C beta 3 Antibody (YA2694)

Phospholipase C beta 3 Antibody (YA2694) Featured Recommendations:

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Phospholipase C beta 3 Antibody (YA2694) Related Classifications

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