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Phospholipase C beta 3 Antibody (YA2694)

(Synonyms: PLCB3; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3)
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Based on 1 Customer Validation

Phospholipase C beta 3 Antibody (YA2694) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospholipase C beta 3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospholipase C beta 3 Antibody (YA2694)
  • Phospholipase C beta 3 Antibody (YA2694) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Phospholipase C beta 3 Antibody (YA2694) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospholipase C beta 3.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 150 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 139 kDa
Species Reactivity Database

Entrez Gene: 5331 Human

SwissProt: Q01970 Human

Immunogen

A synthesized peptide derived from human PLCB3 aa1165-1200.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105068

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospholipase C beta 3 Antibody (YA2694)
    Western blot analysis of extracts from A431(lane 2(20μg)) ,Hela (lane 3(20μg)) and HepG2(lane 4(20μg)) using Phospholipase C beta 3 (HY-P82949) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Phospholipase C beta 3 catalyzes the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Key transducer of G protein-coupled receptor signaling: activated by G(q)/G(11) G alpha proteins downstream of G protein-coupled receptors activation. In neutrophils, participates in a phospholipase C-activating N-formyl peptide-activated GPCR (G protein-coupled receptor) signaling pathway by promoting RASGRP4 activation by DAG, to promote neutrophil functional responses[1][2][3][4].

  • Subcellular Localization

    Cytoplasm; Membrane; Nucleus

  • Isoforms & Post-Translational Modification

    Q01970 has 2 isomers: Q01970-1: 138799 Da (predicted); Q01970-2: 131205 Da (predicted).

  • Subunit

    Interacts with SHANK2 (By similarity). Interacts with LPAR2 (PubMed:15143197)

  • SwissProt ID

    Q01970

  • Gene ID
    5331 [NCBI]
  • Synonyms

    PLCB3; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3

  • Research Field

    Signal Transduction

Phospholipase C beta 3 Antibody (YA2694) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20μg)) ,Hela (lane 3(20μg)) and HepG2(lane 4(20μg)) using Phospholipase C beta 3 (HY-P82949) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospholipase C beta 3 Antibody (YA2694) Product Image
    1/16
  • Experimental Validation Results for Phospholipase C beta 3 Antibody (YA2694)
  • Phospholipase C beta 3 Antibody (YA2694) Product Image
  • Experimental Validation Results for Phospholipase C beta 3 Antibody (YA2694)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20μg)) ,Hela (lane 3(20μg)) and HepG2(lane 4(20μg)) using Phospholipase C beta 3 (HY-P82949) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospholipase C beta 3 Antibody (YA2694)
  • Phospholipase C beta 3 Antibody (YA2694) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from A431(lane 2(20μg)) ,Hela (lane 3(20μg)) and HepG2(lane 4(20μg)) using Phospholipase C beta 3 (HY-P82949) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospholipase C beta 3 Antibody (YA2694) Product Image WB