Glucose 6 phosphate isomerase Antibody (YA9903)
(Synonyms: GPI; Glucose-6-phosphate isomerase; GPI; Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI; Sperm antigen 36; SA-36)
Based on 1 Customer Validation
Glucose 6 phosphate isomerase Antibody (YA9903) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Glucose 6 phosphate isomerase.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, FC, ICC/IF, IF-Tissue
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Reactivity :
Human
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Formulation:
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:50-200 | 1:50-200 | 1:50-200 | 1:50-100 |
Product Details
Glucose 6 phosphate isomerase Antibody (YA9903) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Glucose 6 phosphate isomerase.
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Host Rabbit
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 54 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 63 kDa
Recombinant protein within Human Glucose 6 phosphate isomerase aa 1-128 / 558.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg), PC-3 (lane4, 20μg) and MCF-7 (lane5, 20μg) using Glucose 6 phosphate isomerase Antibody (HY-P810641). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
Background
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Function
Glucose 6 phosphate isomerase is an Isomerase that catalyzes the conversion of alpha-D-glucose-6-phosphate to beta-D-fructose-6-phosphate, the second step in glycolysis, and the reverse reaction in gluconeogenesis, within the cytoplasm. Also shows C2-epimerase activity, interconverting D-glucose-6-phosphate (G6P) and D-mannose-6-phosphate (M6P) (By similarity). Also displays anomerase activity, interconverting alpha and beta-anomeric forms of G6P, D-fructose-6-phosphate and M6P (By similarity). In addition to its metabolic role, this enzyme functions extracellularly as a cytokine: acts as autocrine motility factor (AMF), a secreted angiogenic factor that enhances endothelial cell motility. Functions as neuroleukin, a neurotrophic factor supporting the survival of spinal and sensory neurons. Released by lectin-stimulated T-cells to induce immunoglobulin secretion[1][2][3][4].
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Subcellular Localization
Cytoplasm; Secreted
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Isoforms & Post-Translational Modification
Glucose 6 phosphate isomerase has 2 isoforms, P06744-1: amino acid length is 558, molecular weight is 63147 Da (predicted); P06744-2: amino acid length is 569, molecular weight is 64325 Da (predicted).
Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway. -
Subunit
Homodimer; in the catalytically active form.
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SwissProt ID
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Synonyms
GPI; Glucose-6-phosphate isomerase; GPI; Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI; Sperm antigen 36; SA-36
Documentation
References
[1]. Mojzikova R, et al. Two novel mutations (p.(Ser160Pro) and p.(Arg472Cys)) causing glucose-6-phosphate isomerase deficiency are associated with erythroid dysplasia and inappropriately suppressed hepcidin. Blood Cells Mol Dis. 2018 Mar;69:23-29. [Content Brief]
[2]. Funasaka T, et al. Tumor autocrine motility factor is an angiogenic factor that stimulates endothelial cell motility. Biochem Biophys Res Commun. 2001 Jul 6;285(1):118-28. [Content Brief]
[3]. Haga A, et al. Phosphohexose isomerase/autocrine motility factor/neuroleukin/maturation factor is a multifunctional phosphoprotein. Biochim Biophys Acta. 2000 Jul 14;1480(1-2):235-44. [Content Brief]
[4]. Faik P, et al. Mouse glucose-6-phosphate isomerase and neuroleukin have identical 3' sequences. Nature. 1988 Mar 31;332(6163):455-7. [Content Brief]
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