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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. DOPA Decarboxylase Antibody (YA2428)

DOPA Decarboxylase Antibody (YA2428)

Cat. No.: HY-P82683
COA
SDS
User Guide for Antibodies Technical Support

DOPA Decarboxylase Antibody (YA2428) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DOPA Decarboxylase.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • Descripciòn

Descripciòn

DOPA Decarboxylase Antibody (YA2428) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DOPA Decarboxylase.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Peso molecular
Predicted band size: 54 kDa;
Observed band size: 54 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human DOPA Decarboxylase aa40-95/480.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Pureza Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from HEK293T(lane 2(20ug) , RAW264.7(lane 3(20ug) and HepG2(lane 4(20ug)using DOPA Decarboxylase Antibody (HY-P82683) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine and L-5-hydroxytryptophan to serotonin
Expression：
Tissue_specificity:High expression in kidney
Isoforms & Post-Translational Modification：P20711 has 4 isomers: P20711-1: 53926 Da (predicted); P20711-2: 37118 Da (predicted); P20711-3: 45479 Da (predicted); P20711-4: 44239 Da (predicted).
Subunit：Homodimer
RRID
Database

Entrez Gene: 1644 Human ; 13195 Mouse ; 24311 Rat

SwissProt: P20711 Human ; O88533 Mouse ; P14173 Rat

OMIM: 608643 Human

Research Field

Neuroscience
Synonyms
AADC; Aromatic L Amino Acid Decarboxylase; DDC; DOPA decarboxylase (aromatic L-amino acid decarboxylase); DOPA decarboxylase
Documentación
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

DOPA Decarboxylase Antibody (YA2428) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:
DOPA Decarboxylase Antibody (YA2428)
Cat. No.:
HY-P82683
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