2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. C-erbB-2/HER2 Antibody (YA771)(PBS only)

C-erbB-2/HER2 Antibody (YA771)(PBS only)

製品番号: HY-P80658A
User Guide for Antibodies Technical Support

C-erbB-2/HER2 Antibody (YA771) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to C-erbB-2/HER2.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

C-erbB-2/HER2 Antibody (YA771) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to C-erbB-2/HER2.
Host

Mouse
分子量
Predicted band size: 138 kDa;
Observed band size: 180 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to ErbB 2.The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
純度 affinity purified Conjugation Non-conjugated
Isotype IgG1  
Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization; In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth
Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell projection, ruffle membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Early endosome; Cytoplasm, perinuclear region; Nucleus; Cytoplasm; Nucleus; Cytoplasm; Nucleus
Expression：
Tissue_specificity:This gene is expressed in a variety of tumor tissues, including primary breast tumors as well as tumors of the small intestine, esophagus, kidneys, and oral cavity.
Isoforms & Post-Translational Modification：P04626 has 6 isomers: P04626-1: 137910 Da (predicted); P04626-2: 70917 Da (predicted); P04626-3: 62568 Da (predicted); P04626-4: 136501 Da (predicted); P04626-5: 134856 Da (predicted); P04626-6: 97382 Da (predicted).
Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172, PubMed:33497358). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172)
Subunit：Homodimer (PubMed:21454582). Heterodimer with EGFR, ERBB3 and ERBB4 (PubMed:10358079, PubMed:15093539, PubMed:16978839, PubMed:21190959).
Research Field

Cancer
Synonyms
ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD antigen CD340
ドキュメンテーション
User Guide for Antibodies (1077 KB)

C-erbB-2/HER2 Antibody (YA771)(PBS only) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
C-erbB-2/HER2 Antibody (YA771)(PBS only)
製品番号:
HY-P80658A
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