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DLC1 Antibody

(Synonyms: ARHGAP7, KIAA1723, STARD12, DLC1, Rho GTPase-activating protein 7, Deleted in liver cancer 1 protein, HP protein, Rho-type GTPase-activating protein 7, START domain-containing protein 12, StAR-related lipid transfer protein 12, DLC-1, StARD12)

DLC1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to DLC1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • DLC1 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-2000 1:100-200 1:50-200

Product Details

Description

DLC1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to DLC1.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 170 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 170 kDa
Immunogen

Synthetic peptide corresponding to the N-term region of human DLC1.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    DLC1 functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality. Required for growth factor-induced epithelial cell migration; in resting cells, interacts with TNS3 while PTEN interacts with the p85 regulatory subunit of the PI3K kinase complex but growth factor stimulation induces phosphorylation of TNS3 and PTEN, causing them to change their binding preference so that PTEN interacts with DLC1 and TNS3 interacts with p85. The PTEN-DLC1 complex translocates to the posterior of migrating cells to activate RHOA while the TNS3-p85 complex translocates to the leading edge of migrating cells to promote RAC1 activation[1].

  • Subcellular Localization

    Cytoplasm; Cell junction, focal adhesion; Membrane

  • Expression


    Tissue_Specificity: Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.

  • Isoforms & Post-Translational Modification

    DLC1 has 6 isoforms, Q96QB1-2: amino acid length is 1528, molecular weight is 170591 Da (predicted); Q96QB1-1: amino acid length is 1091, molecular weight is 122827 Da (predicted); Q96QB1-3: amino acid length is 498, molecular weight is 54552 Da (predicted); Q96QB1-4: amino acid length is 1017, molecular weight is 114152 Da (predicted); Q96QB1-5: amino acid length is 463, molecular weight is 50890 Da (predicted); Q96QB1-6: amino acid length is 1125, molecular weight is 126746 Da (predicted).DLC1 存在 6 个异构体，Q96QB1-2：氨基酸个数为 1528 个，分子量为 170591 Da (预测)；Q96QB1-1：氨基酸个数为 1091 个，分子量为 122827 Da (预测)；Q96QB1-3：氨基酸个数为 498 个，分子量为 54552 Da (预测)；Q96QB1-4：氨基酸个数为 1017 个，分子量为 114152 Da (预测)；Q96QB1-5：氨基酸个数为 463 个，分子量为 50890 Da (预测)；Q96QB1-6：氨基酸个数为 1125 个，分子量为 126746 Da (预测)。

  • Subunit

    Interacts with EF1A1, facilitates EF1A1 distribution to the membrane periphery and ruffles upon growth factor stimulation and suppresses cell migration.

  • SwissProt ID

    Q96QB1

  • Gene ID
    10395 [NCBI]
  • Synonyms

    ARHGAP7, KIAA1723, STARD12, DLC1, Rho GTPase-activating protein 7, Deleted in liver cancer 1 protein, HP protein, Rho-type GTPase-activating protein 7, START domain-containing protein 12, StAR-related lipid transfer protein 12, DLC-1, StARD12

[1]. Cao X, et al. A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migration. Nat Commun. 2015 Jul 13;6:7721. [Content Brief]

DLC1 Antibody Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • DLC1 Antibody Product Image